Bovine Interleukin Array (11 targets) (SARB0004)

SKU:
SARB0004
€514 - €993

Description

Bovine Interleukin Array Product Information

Product Sku:SARB0004
Size:2, 4, or 8
Species Detected:Bovine
Number of Targets Detected:11
Gene Symbols:IL10, IL13, IL15, IL17A, IL18, IL1A, IL1B, IL2, IL21, IL36RN|IL4
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Bovine Interleukin Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

   

IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 281250
  • Accession #: P08831
IL-1 beta (IL-1 F2)
  • Gene Symbol: IL1B
  • Gene ID: 281251
  • Accession #: P09428
IL-36 Ra (IL-1 F5)
  • Gene Symbol: IL36RN FIL1D IL1F5 IL1HY1 IL1L1 IL1RP3 UNQ1896/PRO4342
IL-10
  • Gene Symbol: IL10
  • Gene ID: 281246
  • Accession #: P43480
IL-13
  • Gene Symbol: IL13
  • Gene ID: 281247
  • Accession #: Q9XSV9
IL-15
  • Gene Symbol: IL15
  • Gene ID: 281248
  • Accession #: Q28028
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 282863
  • Accession #: Q687Y7
IL-18
  • Gene Symbol: IL18 IGIF
  • Gene ID: 281249
  • Accession #: Q9TU73
IL-2
  • Gene Symbol: IL2 IL-2
  • Gene ID: 280822
  • Accession #: P05016
IL-21
  • Gene Symbol: IL21
  • Gene ID: 378475
  • Accession #: Q76LU5
IL-4
  • Gene Symbol: IL4
  • Gene ID: 280824
  • Accession #: P30367
    

Bovine Interleukin Array Kit Components

  • Bovine Interleukin Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Bovine Interleukin Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-36 Ra (IL-1 F5)
Target:
IL-10
Target:
IL-13
Target:
IL-15
Target:
IL-17A
Target:
IL-18
Target:
IL-2
Target:
IL-21
Target:
IL-4
Reactivity:
Bovine
Application:
Multiplex Array
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