Human BMP Related Array (8 targets) (SARB0026)

SKU:
SARB0026
€422 - €808

Description

Human BMP Related Array Product Information

Product Sku:SARB0026
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:8
Gene Symbols:BMP2, BMP4, BMP5, BMP6, BMP7, BMP8A, GDF11, GDF2
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human BMP Related Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

GDF-11 (BMP-11)
  • Gene Symbol: GDF11 BMP11
  • Gene ID: 10220
  • Accession #: O95390
BMP-2
  • Gene Symbol: BMP2 BMP2A
  • Gene ID: 650
  • Accession #: P12643
BMP-4
  • Gene Symbol: BMP4 BMP2B DVR4
  • Gene ID: 652
  • Accession #: P12644
BMP-5
  • Gene Symbol: BMP5
  • Gene ID: 653
  • Accession #: P22003
BMP-6
  • Gene Symbol: BMP6 VGR
  • Gene ID: 654
  • Accession #: P22004
BMP-7
  • Gene Symbol: BMP7 OP1
  • Gene ID: 655
  • Accession #: P18075
BMP-8
  • Gene Symbol: BMP8A
  • Gene ID: 353500
  • Accession #: Q7Z5Y6
BMP-9
  • Gene Symbol: GDF2 BMP9
  • Gene ID: 2658
  • Accession #: Q9UK05
  

Human BMP Related Array Kit Components

  • Human BMP Related Array 2
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human BMP Related Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
GDF-11 (BMP-11)
Target:
BMP-2
Target:
BMP-4
Target:
BMP-5
Target:
BMP-6
Target:
BMP-7
Target:
BMP-8
Target:
BMP-9
Reactivity:
Human
Application:
Multiplex Array
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