Human Obesity Array (62 targets) (SARB0015)

SKU:
SARB0015
€644 - €1,297

Description

Human Obesity Array Product Information

Product Sku:SARB0015
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:62
Gene Symbols:ACE2, ADIPOQ, AGRP, ANGPT1, ANGPT2, ANGPTL4, CCL16, CCL18, CCL2, CCL25|CCL4, CCL5, CCL7, CFD, CRP, CSF1, CXCL10, CXCL12, CXCL5, CXCL8, EDA2R, FAS, FGF6, GH1, IFNG, IGF1, IGF1R, IGFBP1, IGFBP2, IGFBP3, IL10, IL11, IL12A, IL1A, IL1B, IL1R1, IL1RL1, IL6, IL6R, INS, LEP, LEPR, LIF, MIF, MST1, OSM, PDGFA, PDGFB, RETN, SAA1, SERPINE1, TGFB1, TIMP1, TIMP2, TNF, TNFRSF11B, TNFRSF1A, TNFRSF1B, TNFRSF9, TNFSF9, VEGFA, XCL1
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Obesity Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

4-1BB (TNFRSF9/CD137)ACE-2Adiponectin (ACRP30)Adipsin (Complement Factor D)AgRP
Angiopoietin-1Angiopoietin-2ANGPTL4CRP (C-Reactive Protein)ENA-78 (CXCL5)
Fas (TNFRSF6/Apo-1)FGF-6Growth HormoneHCC-4 (CCL16)IFN-gamma
IGFBP-1IGFBP-2IGFBP-3IGF-1IGF-1 R
IL-1 R4 (ST2)IL-1 R1IL-10IL-11IL-12 p70
IL-1 alpha (IL-1 F1)IL-1 beta (IL-1 F2)IL-6IL-6 RIL-8 (CXCL8)
InsulinIP-10 (CXCL10)Leptin RLeptinLIF
Lymphotactin (XCL1)MCP-1 (CCL2)MCP-3 (MARC/CCL7)M-CSFMIF
MIP-1 beta (CCL4)MSP alpha/betaOsteoprotegerin (TNFRSF11B)Oncostatin MPAI-1
PARC (CCL18)PDGF-AAPDGF-ABPDGF-BBRANTES (CCL5)
ResistinSAA (Serum Amyloid A)SDF-1 alpha (CXCL12 alpha)TNF RII (TNFRSF1B)TNF RI (TNFRSF1A)
TECK (CCL25)TGF beta 1TIMP-1TIMP-2TNF alpha
VEGF-AXEDAR   

Human Obesity Array Kit Components

  • Human Obesity Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Obesity Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
4-1BB (TNFRSF9/CD137)
Target:
ACE-2
Target:
Adiponectin (ACRP30)
Target:
Adipsin (Complement Factor D)
Target:
AgRP
Target:
Angiopoietin-1
Target:
Angiopoietin-2
Target:
ANGPTL4
Target:
CRP (C-Reactive Protein)
Target:
ENA-78 (CXCL5)
Target:
Fas (TNFRSF6/Apo-1)
Target:
FGF-6
Target:
Growth Hormone
Target:
HCC-4 (CCL16)
Target:
IFN-gamma
Target:
IGFBP-1
Target:
IGFBP-2
Target:
IGFBP-3
Target:
IGF-1
Target:
IGF-1 R
Target:
IL-1 R4 (ST2)
Target:
IL-1 R1
Target:
IL-10
Target:
IL-11
Target:
IL-12 p70
Target:
IL-1 alpha (IL-1 F1)
Target:
IL-1 beta (IL-1 F2)
Target:
IL-6
Target:
IL-6 R
Target:
IL-8 (CXCL8)
Target:
Insulin
Target:
IP-10 (CXCL10)
Target:
Leptin R
Target:
Leptin
Target:
LIF
Target:
Lymphotactin (XCL1)
Target:
MCP-1 (CCL2)
Target:
MCP-3 (MARC/CCL7)
Target:
M-CSF
Target:
MIF
Target:
MIP-1 beta (CCL4)
Target:
MSP alpha/beta
Target:
Osteoprotegerin (TNFRSF11B)
Target:
Oncostatin M
Reactivity:
Human
Application:
Multiplex Array
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