Human NF-kB Array (34 targets) (SARB0060)

SKU:
SARB0060
€644 - €1,297

Description

Human NF-kB Array Product Information

Product Sku:SARB0060
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:34
Gene Symbols:ASC, BCL10, BIRC2, BIRC3, CD40, CIS4, FADD, FAS, FIP3, IKBB|IKBE, IKBKB, IKBKE, IL17RA, IL18R1, IL1R1, IL1RN, IRAK, IRF5, JNK2, LTBR, LYT10, MYD88, NFKB1, NGFR, REL, TLR2, TMEM173, TNFRSF10A, TNFRSF10B, TNFRSF1A, TNFRSF1B, TP53
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:NFkB Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human NF-kB Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

ASC (PYCARD)BCL-10CD40 (TNFRSF5)cIAP-1 (BIRC2/hIAP-2)cIAP-2
c-RelFADDFas (TNFRSF6/Apo-1)IkB-alphaIKB-b (NFKBIB)
IkB-epsilon (NF-kappa-BIE)IKK-BIKK-E (IKBKE)IKK-gamma (NEMO/FIP-3)IL-1 Ra (IL-1 F3)
IL-1 R1IL-17 RAIL-1 R5 (IL-18 R alpha)IRAK1IRF5
JNK2 (MAPK9 isoform alpha-1)Lymphotoxin beta R (TNF RIII)MyD88NFKB1nfkb2
NGFR (TNFRSF16)p53socs6STINGTLR2
TNF RI (TNFRSF1A)TNF RII (TNFRSF1B)TRAIL R1 (TNFRSF10A/DR4)TRAIL R2 (TNFRSF10B/DR5) 

Human NF-kB Array Kit Components

  • Human NF-kB Array C2 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 1,000X HRP-Anti-Rabbit-IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or plastic Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system: CCD camera, X-ray Film and a suitable film processor, gel documentation system, or another chemiluminescent detection system capable of imaging a western blot.

Human NF-kB Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
View AllClose

Additional Information

Product type:
Protein Array
Target:
ASC (PYCARD)
Target:
BCL-10
Target:
CD40 (TNFRSF5)
Target:
cIAP-1 (BIRC2/hIAP-2)
Target:
cIAP-2
Target:
c-Rel
Target:
FADD
Target:
Fas (TNFRSF6/Apo-1)
Target:
IkB-alpha
Target:
IKB-b (NFKBIB)
Target:
IkB-epsilon (NF-kappa-BIE)
Target:
IKK-B
Target:
IKK-E (IKBKE)
Target:
IKK-gamma (NEMO/FIP-3)
Target:
IL-1 Ra (IL-1 F3)
Target:
IL-1 R1
Target:
IL-17 RA
Target:
IL-1 R5 (IL-18 R alpha)
Target:
IRAK1
Target:
IRF5
Target:
JNK2 (MAPK9 isoform alpha-1)
Target:
Lymphotoxin beta R (TNF RIII)
Target:
MyD88
Target:
NFKB1
Target:
nfkb2
Target:
NGFR (TNFRSF16)
Target:
p53
Target:
socs6
Target:
STING
Target:
TLR2
Target:
TNF RI (TNFRSF1A)
Target:
TNF RII (TNFRSF1B)
Target:
TRAIL R1 (TNFRSF10A/DR4)
Target:
TRAIL R2 (TNFRSF10B/DR5)
Reactivity:
Human
Application:
Multiplex Array
View AllClose