Human Cytokine Array (54 targets) (SARB0043)

SKU:
SARB0043
€644 - €1,297

Description

Human Cytokine Array Product Information

Product Sku:SARB0043
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:54
Gene Symbols:ALCAM, BMP5, BMP7, CCL23, CD14, CD80, CDH5, CSF1R, CTF1, CXCL10|CXCL12, CXCL16, ENG, ERBB3, FASLG, FLT4, ICAM2, IGF2, IL10RB, IL13RA2, IL18BP, IL18RAP, IL1R2, IL21R, IL2RB, IL2RG, IL5RA, IL9, INHBA, KDR, KIT, LEPR, LIF, MMP1, MMP13, MMP3, MMP9, NGFR, PDGFA, PDGFB, PDGFRA, PDGFRB, PECAM1, PRL, SELE, SELL, SIGLEC5, TEK, TGFA, TGFB1, TGFB2, TIE1, TIMP4, TNFRSF21
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway: 
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Human Cytokine Array Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names:

Activin AALCAM (CD166)CD80 (B7-1)BMP-5BMP-7
Cardiotrophin-1 (CT-1)CD14CXCL16DR6 (TNFRSF21)Endoglin (CD105)
ErbB3E-SelectinFas Ligand (TNFSF6)ICAM-2 (CD102)IGF-2
IL-1 R2IL-10 R betaIL-13 R alpha 2IL-18 BP alphaIL-18 R beta (AcPL)
MMP-3IL-2 R beta (CD122)IL-2 R gamma (Common gamma Chain)IL-21 RIL-5 R alpha
IL-9IP-10 (CXCL10)LAP/TGF beta 1Leptin RLIF
L-Selectin (CD62L)M-CSF RMMP-1MMP-13MMP-9
MPIF-1 (CCL23)NGFR (TNFRSF16)PDGF-AAPDGF-ABPDGF R alpha
PDGF R betaPECAM-1 (CD31)ProlactinSCF R (CD117/c-kit)SDF-1 beta (CXCL12 beta)
Siglec-5 (CD170)TGF alphaTGF beta 2Tie-1Tie-2
TIMP-4VE-Cadherin (CDH5)VEGFR2VEGFR3 

Human Cytokine Array Kit Components

  • Human Cytokine Antibody Array C8 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Human Cytokine Array Protocol Outline

1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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Additional Information

Product type:
Protein Array
Target:
Activin A
Target:
ALCAM (CD166)
Target:
CD80 (B7-1)
Target:
BMP-5
Target:
BMP-7
Target:
Cardiotrophin-1 (CT-1)
Target:
CD14
Target:
CXCL16
Target:
DR6 (TNFRSF21)
Target:
Endoglin (CD105)
Target:
ErbB3
Target:
E-Selectin
Target:
Fas Ligand (TNFSF6)
Target:
ICAM-2 (CD102)
Target:
IGF-2
Target:
IL-1 R2
Target:
IL-10 R beta
Target:
IL-13 R alpha 2
Target:
IL-18 BP alpha
Target:
IL-18 R beta (AcPL)
Target:
MMP-3
Target:
IL-2 R beta (CD122)
Target:
IL-2 R gamma (Common gamma Chain)
Target:
IL-21 R
Target:
IL-5 R alpha
Target:
IL-9
Target:
IP-10 (CXCL10)
Target:
LAP/TGF beta 1
Target:
Leptin R
Target:
LIF
Target:
L-Selectin (CD62L)
Target:
M-CSF R
Target:
MMP-1
Target:
MMP-13
Target:
MMP-9
Target:
MPIF-1 (CCL23)
Target:
NGFR (TNFRSF16)
Target:
PDGF-AA
Target:
PDGF-AB
Target:
PDGF R alpha
Target:
PDGF R beta
Target:
PECAM-1 (CD31)
Target:
Prolactin
Target:
SCF R (CD117/c-kit)
Reactivity:
Human
Application:
Multiplex Array
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