Sucrose Assay Kit (Colorimetric) (BA0151)

Sucrose Assay Kit

Sucrose (C₁₂H₂₂O₁₁) is a disaccharide of glucose and fructose with an alpha-1,2-glycosidic linkage. It is the most common food sweetener and the most important sugar in plants. In mammals, sucrose is readily digested in the stomach into glucose and fructose, which are rapidly absorbed into the bloodstream in the small intestine. Simple, direct and high-throughput assays for measuring sucrose concentrations find wide applications.

Assay Genie's improved sucrose assay uses invertase to digest sucrose into fructose and glucose. The resulting fructose is then quantified using our fructose assay reagent. The measured color intensity at 565nm is directly proportional to the sucrose concentration in the sample.

Applications

For quantitative determination of sucrose.

Key Features

• No interference by glucose.
• Use 20 uL samples. Linear detection range: 17 to 2000 uM sucrose.

Sucrose Assay Kit Information

Kit Includes:	Assay Buffer: 10 mL, Invertase: 120 mL, PMS Solution: 1.5 mL, Enzyme: Dried MTT, Solution: 1.5 mL, Enzyme Buffer: 150 mL, Standard: 400 mL, 40 mM Sucrose
Kit Requires:	Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader.
Method of Detection:	OD565nm
Detection Limit:	17 uM
Samples:	Serum, plasma, urine, saliva, milk, culture medium, food, beverage, agriculture etc
Species:	All
Protocol Length:	70 min
Size:	100 tests
Storage:	Store all components at -20°C.
Shelf Life:	6 months