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Glucose Assay Kit - InformatoionAssay Genie's glucose assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to glucose concentration in the sample.
ApplicationsFor quantitative determination of glucose and evaluation of drug effects on glucose metabolism.
Glucose Assay Kit - Key features
- Sensitive and accurate. Use as little as 20 samples. Linear detection range in 96-well plate: 5 to 300 (90 /dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 for fluorimetric assays.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
|Kit Includes||Assay Buffer: 10 mL Enzyme Mix: 120 mL Dye Reagent: 120 mL Standard: 1 mL 300 mg/dL Glucose|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader.|
|Method of Detection||OD570nm, or FL530/585nm|
|Samples||Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc|
|Protocol Length||40 min|
|Storage||Store Assay Buffer at 4°C and other components at -20°C|
|Shelf Life||6 months|