Glucose Assay Kit (BA0081)

Glucose Assay Kit - Informatoion

Assay Genie's glucose assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to glucose concentration in the sample.

Applications

For quantitative determination of glucose and evaluation of drug effects on glucose metabolism.

Glucose Assay Kit - Key features

• Sensitive and accurate. Use as little as 20 samples. Linear detection range in 96-well plate: 5 to 300 (90 /dL to 5.4 mg/dL) glucose for colorimetric assays and 1 to 30 for fluorimetric assays.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.

Kit Includes	Assay Buffer: 10 mL Enzyme Mix: 120 mL Dye Reagent: 120 mL Standard: 1 mL 300 mg/dL Glucose
Kit Requires	Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader.
Method of Detection	OD570nm, or FL530/585nm
Detection Limit	5
Samples	Serum, plasma, urine, saliva, milk, culture medium, food, agriculture etc
Species	All
Protocol Length	40 min
Size	100 tests
Storage	Store Assay Buffer at 4°C and other components at -20°C
Shelf Life	6 months

More Details

Glucose (C₆H₁₂O₆) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, hyperactivity of thyroid, pituitary and adrenal glands. Decreased levels are found in insulin secreting tumors, myxedema, hypopituitarism and hypoadrenalism. Simple, direct and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery.