Glucose Oxidase Assay Kit
Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery.
Assay Genie's glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to glucose oxidase activity in the sample.
For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
- Sensitive and accurate. Use as little as 20 uL samples. Linear detection range in 96-well plate for 20 minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
Glucose Oxidase Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL HRP Enzyme: 120 uL Glucose: 1.5 mL 2 M Glucose Dye Reagent: 120 uL Standard: 100 uL 3% H2O2|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader.|
|Method of Detection:||OD570nm, FL530/585nm|
|Detection Limit:||0.02 (OD), 0.002U/L (FL).|
|Samples:||Cell/tissue lysate, cell culture media etc|
|Protocol Length:||20 min|
|Storage:||Store assay buffer at 4°C and all other reagents at -20°C|
|Shelf Life:||6 months|