Monoamine Oxidase Assay Kit
Monoamine oxidases (MAO, EC 188.8.131.52) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.
Assay Genie's Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure monoamine oxidase (MAO) enzyme activity. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (Ex/Em = 530/585nm). The assay is simple, sensitive, stable and high-throughput adaptable.
For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen.
- Safe. Non-radioactive assay.
- Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Monoamine Oxidase Assay Kit Information:
|Kit Includes:||Assay Buffer: 12 mL (pH 7.4), p-Tyramine: 120 mL, Pargyline: 50 mL, 20 mM HRP Enzyme: 120 mL, Clorgyline: 50 mL, 20 mM Dye Reagent: 120 mL, Hydrogen Peroxide: 100 mL 3% H2O2|
|Kit Requires:||Pipetting devices, centrifuge tubes, black flat bottom 96-well plate|
|Method of Detection:||FL530/585nm|
|Detection Limit:||0.01 U/L|
|Samples:||MAO enzyme preparations|
|Protocol Length:||30 min|
|Storage:||Store Assay Buffer and Hydrogen Peroxide at 4°C and other reagents at - 20°C.|
|Shelf Life:||6 months|