Xanthine Oxidase Assay Kit
Xanthine Oxidase catalyzes the oxidation of xanthine to uric acid. In addition, xanthine oxidase can catalyze the oxidation of hypoxanthine to xanthine, act on certain purines and aldehydes, and in certain cases produce the superoxide ion. Clinically, xanthine oxidase activity in blood can act as a marker for influenza, liver damage, and possibly cardiovascular health. Simple, direct and high-throughput assays for measuring xanthine oxidase activity find wide applications in research and drug discovery.Assay Genie's xanthine oxidase assay kit uses a single Working Reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to xanthine oxidase activity in the sample.
ApplicationsFor quantitative determination of xanthine oxidase enzyme activity.
- Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays.
- Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
Xanthine Oxidase Assay Kit Information
|Kit Includes:||Assay Buffer: 10 mL, HRP Enzyme:0 120 uL, Xanthine: 1.5 mL, 5 mM Xanthine Dye ageRent: 12 uL, Standard: 100 uL 3% H2O2|
|Kit Requires:||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, black 96-well plates and plate reader.|
|Method of Detection:||OD570nm, FL530/585nm|
|Detection Limit:||FL 0.01 U/L; OD 0.03 U/L|
|Samples:||Cell lysate, serum, and other biological samples.|
|Protocol Length:||20 min|
|Storage:||Store all reagents at -20°C|
|Shelf Life:||6 months|