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Description
Glycolysis Assay Kit - Information
Assay Genie's Glycolysis assay kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media when glycolysis occurs and is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH which reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to rate of glycolysis in the cells.Applications
Direct determination of L-Lactate produced by glycolysis in cell samples. Screening of glycolysis inhibitors.Glycolysis Assay Kit - Key Features
- Fast and sensitive. Use of 5 uL sample. Linear detection range up to 10 mM L-lactate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
- High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Glycolysis Assay Kit - Data Sheet | |
Kit Includes | Reagent: 12 mL Standard: 250 uL |
Kit Requires | Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader |
Method of Detection | OD565nm |
Detection Limit | NA |
Samples | Cell media |
Species | All |
Protocol Length | 30 min |
Size | 100 tests |
Storage | Store all components at -20°C upon receiving |
Shelf Life | 6 months |