NADP+/NADPH Assay Kit (BA0200)

NADP+/NADPH Assay Kit - Information

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Simple, direct and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. Assay Genies' EnzyChromTM NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH and their ratio.

Key Features

1. Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay.
2. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required.
3. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Application

Direct Assays: NADP+/NADPH concentrations and ratios in cell or tissue extracts.

Method

Colorimetric Determination at 565 nm

Samples

Cells, Tissue

Species

Human, mouse, rat and more

Size

100 tests

Detection Limit

0.1 µM

Shelf Life

6 months