NAD/NADH Assay Kit (Colorimetric) (BA0067)
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NAD/NADH Assay Kit - InformationAssay Genie's NAD/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.
ApplicationsFor sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
NAD/NADH Assay Kit - Key Features
- Sensitive and accurate. Detection limit of 0.05 uM and linearity up to 10 uM NAD+/NADH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
NAD/NADH Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 10 mL Enzyme A: 120 mL Lactate: 1.5 mL Enzyme B: 120 mL MTT Solution: 1.5 mL NAD Standard: 0.5 mL NAD/NADH Extraction Buffers: each 12 mL|
|Kit Requires||Pipetting (multi-channel) devices. Clear-bottom 96-well plates and plate reader.|
|Method of Detection||OD565nm|
|Detection Limit||0.05 uM|
|Samples||Cell or tissue extracts|
|Protocol Length||15 min|
|Storage||Store all reagents at -20°C.|
|Shelf Life||6 months|