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ADP/ATP Ratio Assay Kit
Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis.Assay Genie's ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration. In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.
ApplicationsFor quantitative bioluminescent assay for ADP:ATP ratio (apoptosis) in cells.
- Safe. Non-radioactive assay.
- Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.
- Robust and amenable to HTS: Z' factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
ADP/ATP Ratio Assay Kit Information:
|Kit Includes:||Assay Buffer: 10 mL, Substrate: 120 mL, Cosubstrate: 120 mL, ATP Enzyme: 120 mL, ADP Enzyme: 120 mL|
|Method of Detection:||Luminescence|
|Protocol Length:||20 min|
|Storage:||Store all reagents at -20°C|
|Shelf Life:||12 months|