Arginase Activity Assay Kit (Colorimetric) (BA0016)
Arginase Activity Assay Kit - InformationAssay Genie's arginase assay kit provides a sensitive and convenient method for arginase activity determination. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample.
ApplicationsFor quantitative determination of arginase activity and screen for its inhibitors.
Arginase Activity Assay Kit - Key Features
- Sensitive and accurate. Detection limit: 0.3 U/L for 2 hr arginase reaction in 96-well assay format.
- Simple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day.
Arginase Activity Assay Kit - Data Sheet
|Kit Includes||Arginine Buffer (pH 9.5): 1.5 mL Mn Solution: 300 uL Reagent A: 12 mL Reagent B: 12 mL Urea standard (50 mg/dL): 0.5 mL|
|Kit Requires||Pipetting devices and accessories, clear bottom 96-well plates, plate reader and, for plasma and serum, Amicon Ultra- 0.5, Ultracel-10 Membrane.|
|Method of Detection||OD430nm|
|Detection Limit||1 U/L (0.3 U/L for 2 hr reaction)|
|Samples||Enzyme preparations, serum, plasma, tissue culture etc|
|Protocol Length||2 hrs|
|Storage||. Store the Arginine Buffer and Urea Standard at -20°C, and other components at 2-8°C.|
|Shelf Life||12 months|
More DetailsARGINASE (L-arginine ureohydrolase EC 126.96.36.199) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, completing the last step in the urea cycle. Arginase activity is a key diagnostic indicator. Increased levels of arginase activity in blood have been associated with liver damage. Hyperargininemia due to arginase deficiency is an inherited autosomal recessive disease. Simple, direct and automation-ready procedures for measuring arginase activity in biological samples are highly desirable in Research and Drug Discovery.