GST Activity Assay Kit (Colorimetric) (BA0028)
GST Activity Assay Kit - InformationAssay Genie's Glutathione S-transferase, GST activity assay kit (colorimetric) is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.
ApplicationsGlutathione S-transferase activity determination in biological samples
GST Activity Assay Kit - Key Features
- Fast and sensitive. Linear detection range (20 uL sample): 2 to 80 U/L for 10 min reaction at 25°C.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
GST Activity Assay Kit - Data Sheet
|Kit Includes||Assay Buffer: 25 mL Glutathione: Powder CDNB: 120 uL|
|Kit Requires||Pipetting devices, centrifuge tubes, clear flat-bottom 96-well plates, and plate reader.|
|Method of Detection||OD340nm|
|Detection Limit||2 U/L|
|Samples||Biological samples (e.g. cell lysates, tissues, etc.)|
|Protocol Length||10 min|
|Shelf Life||6 months|
|Storage||Store all components at 4°C upon receiving|
More DetailsGLUTATHIONE TRANSFERASE (GST; EC 220.127.116.11) is a multifunctionalenzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.