Glutamate Assay Kit
Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism and Alzheimer's disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct and automation-ready procedures for measuring glutamate concentration are very desirable.Assay Genie's glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.
ApplicationsFor quantitative determination of glutamate and evaluation of drug effects on glutamate metabolism.
- Sensitive and accurate. Detection limit of 50 uM, linearity up to 2.5 mM glutamate in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Glutamate Assay Kit Information:
|Method of Detection:||OD565nm|
|Detection Limit:||50 uM|
|Samples:||Serum, plasma, urine, cell, tissue etc|
|Protocol Length:||30 min|
|Shelf Life:||6 months|