Description
IkappaB-alpha (Phospho-Ser32/Ser36)Colorimetric Cell-Based ELISA Kit
The IKKalpha/beta Phospho-Ser32/Ser36 Colorimetric Cell-Based ELISA Kit is a powerful tool for researchers looking to study the phosphorylation status of IKKalpha/beta in cell samples. This kit is designed for accurate detection of phosphorylated IKKalpha/beta levels in cell lysates, providing high sensitivity and specificity for reliable and reproducible results.IKKalpha/beta is a critical kinase complex involved in the regulation of inflammatory responses and cell survival. Phosphorylation of IKKalpha/beta at Ser32/Ser36 is essential for its activation and downstream signaling pathways.
Dysregulation of IKKalpha/beta phosphorylation has been implicated in various diseases, including cancer, autoimmune disorders, and inflammatory conditions.By using the IKKalpha/beta Phospho-Ser32/Ser36 Colorimetric Cell-Based ELISA Kit, researchers can gain valuable insights into the phosphorylation status of IKKalpha/beta in their cell samples, helping to further understand the role of this kinase complex in disease progression and potential therapeutic interventions.
| Product Name: | IkappaB-alpha (Phospho-Ser32/Ser36)Colorimetric Cell-Based ELISA Kit |
| Product Code: | CBCAB01551 |
| ELISA Type: | Cell-Based |
| Target: | IkappaB-alpha (Phospho-Ser32/Ser36) |
| Reactivity: | Human, Mouse, Rat |
| Dynamic Range: | > 5000 Cells |
| Detection Method: | Colorimetric 450 nm |
| Format: | 2 x 96-Well Microplates |
The IkappaB-alpha (Phospho-Ser32/Ser36) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect IkB-alpha protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated IkB-alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IkB-alpha phosphorylation.
Qualitative determination of IkappaB-alpha (Phospho-Ser32/Ser36) concentration is achieved by an indirect ELISA format. In essence, IkappaB-alpha (Phospho-Ser32/Ser36) is captured by IkappaB-alpha (Phospho-Ser32/Ser36)-specific primary (1ø) antibodies while the HRP-conjugated secondary (2ø) antibodies bind the Fc region of the 1ø antibody. Through this binding, the HRP enzyme conjugated to the 2ø antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:
| 1. | A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. |
| 2. | Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. |
| Database Information: | Gene ID: 4792, UniProt ID: P25963, OMIM: 164008/612132, Unigene: Hs.81328 |
| Gene Symbol: | NFKBIA |
| Sub Type: | Phospho |
| UniProt Protein Function: | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. |
| NCBI Summary: | This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P25963 |
| NCBI GenInfo Identifier: | 126682 |
| NCBI Gene ID: | 4792 |
| NCBI Accession: | P25963.1 |
| UniProt Secondary Accession: | P25963,B2R8L6, |
| UniProt Related Accession: | P25963 |
| Molecular Weight: | 35,609 Da |
| NCBI Full Name: | NF-kappa-B inhibitor alpha |
| NCBI Synonym Full Names: | NFKB inhibitor alpha |
| NCBI Official Symbol: | NFKBIA |
| NCBI Official Synonym Symbols: | IKBA; MAD-3; NFKBI |
| NCBI Protein Information: | NF-kappa-B inhibitor alpha |
| UniProt Protein Name: | NF-kappa-B inhibitor alpha |
| UniProt Synonym Protein Names: | I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3 |
| Protein Family: | NF-kappa-B inhibitor |
| UniProt Gene Name: | NFKBIA |
| UniProt Entry Name: | IKBA_HUMAN |
| Component | Quantity |
| 96-Well Cell Culture Clear-Bottom Microplate | 2 plates |
| 10X TBS | 24 mL |
| Quenching Buffer | 24 mL |
| Blocking Buffer | 50 mL |
| 15X Wash Buffer | 50 mL |
| Primary Antibody Diluent | 12 mL |
| 100x Anti-Phospho Target Antibody | 60 µL |
| 100x Anti-Target Antibody | 60 µL |
| Anti-GAPDH Antibody | 60 µL |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12 mL |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12 mL |
| SDS Solution | 12 mL |
| Stop Solution | 24 mL |
| Ready-to-Use Substrate | 12 mL |
| Crystal Violet Solution | 12 mL |
| Adhesive Plate Seals | 2 seals |
The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing ≥1 ml
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
- Orbital shaker (optional)
- Deionized or sterile water
*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1. | Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37 °C prior to adding cells. |
| 2. | Incubate the cells for overnight at 37 °C, 5% CO2. |
| 3. | Treat the cells as desired. |
| 4. | Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice. |
| 5. | Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells. |
| 6. | Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4 °C for a week. |
| 7. | Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature. |
| 8. | Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time. |
| 9. | Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature. |
| 10. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 11. | Add 50 µL of 1x primary antibodies Anti-IkappaB-alpha (Phospho-Ser32/Ser36) Antibody, Anti-IkappaB-alpha Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4 °C. If the target expression is known to be high, incubate for 2 hours at room temperature. |
| 12. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 13. | Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature. |
| 14. | Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time. |
| 15. | Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark. |
| 16. | Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader. |
(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)
