Description
ERF Transcription Factor Activity Assay
The ERF Transcription Factor Activity Assay Kit is a reliable and sensitive tool designed for the quantitative measurement of ERF (ethylene response factor) transcription factor activity in various samples including cell lysates, serum, and tissue homogenates. This kit provides accurate and reproducible results, making it ideal for studying the role of ERF in gene expression regulation and its involvement in various biological processes. ERF is a key transcription factor that plays a crucial role in plant defense responses, stress signaling, and development. Its activity is essential for modulating gene expression in response to environmental cues, ultimately affecting plant growth and adaptation.
The ERF Transcription Factor Activity Assay Kit offers a valuable tool for researchers to explore the function of ERF and its impact on plant physiology, immunity, and stress tolerance. With its user-friendly protocol and high performance, the ERF Transcription Factor Activity Assay Kit is a valuable asset for research labs and biotech companies interested in unraveling the intricate mechanisms of gene regulation and transcriptional control in plants. Invest in this kit today and uncover the hidden secrets of ERF-mediated gene expression pathways.
| Product Name: | ERF Transcription Factor Activity Assay |
| Product Code: | TFAB00097 |
| Target: | ERF |
| Reactivity: | Human, Mouse |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ERF transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ERF: potent transcriptional repressor that binds to the H1 element of the ETS2 promoter. Belongs to the ETS family of transcription factors. Its function is regulated by phosphorylation. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19q13 Cellular Component: cytosol; Golgi apparatus; nucleoplasm; nucleus Molecular Function:sequence-specific DNA binding; transcription corepressor activity Biological Process: cell cycle; cell differentiation; negative regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent Disease: Craniosynostosis 4 |
| NCBI Summary: | ETS2 is a transcription factor and protooncogene involved in development, apoptosis, and the regulation of telomerase. The protein encoded by this gene binds to the ETS2 promoter and is a strong repressor of ETS2 transcription. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P50548 |
| NCBI GenInfo Identifier: | 50403684 |
| NCBI Gene ID: | 2077 |
| NCBI Accession: | P50548.2 |
| UniProt Secondary Accession: | P50548,Q59G38, Q9UPI7, B2RAP1, B7Z4R0, |
| UniProt Related Accession: | P50548 |
| Molecular Weight: | 49,965 Da |
| NCBI Full Name: | ETS domain-containing transcription factor ERF |
| NCBI Synonym Full Names: | ETS2 repressor factor |
| NCBI Official Symbol: | ERF |
| NCBI Official Synonym Symbols: | PE2; CRS4; PE-2 |
| NCBI Protein Information: | ETS domain-containing transcription factor ERF |
| UniProt Protein Name: | ETS domain-containing transcription factor ERF |
| UniProt Synonym Protein Names: | Ets2 repressor factor; PE-2 |
| Protein Family: | Essential recombination function protein |
| UniProt Gene Name: | ERF |
| UniProt Entry Name: | ERF_HUMAN |
