Description
SP1 Transcription Factor Activity Assay
The SP1 Transcription Factor Activity Assay Kit from Assay Genie is a powerful tool for measuring the activity of the SP1 transcription factor in a variety of biological samples. This kit offers high sensitivity and specificity, allowing for accurate and reliable results that are essential for understanding the role of SP1 in gene regulation.SP1 is a key transcription factor that plays a critical role in regulating the expression of genes involved in cell growth, differentiation, and apoptosis. dysregulation of SP1 activity has been linked to various diseases, including cancer and inflammatory disorders.
By accurately measuring SP1 activity, researchers can gain valuable insights into the molecular mechanisms underlying disease development and progression.The SP1 Transcription Factor Activity Assay Kit is easy to use and provides quick results, making it suitable for high-throughput screening studies and basic research applications. With its high quality and performance, this kit is a must-have for any laboratory studying transcriptional regulation and its impact on human health.
| Product Name: | SP1 Transcription Factor Activity Assay |
| Product Code: | TFAB00160 |
| Target: | SP1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie SP1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease. |
| UniProt Protein Details: | Protein type:DNA-binding; C2H2-type zinc finger protein; Transcription factor Chromosomal Location of Human Ortholog: 12q13.1 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein C-terminus binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor binding; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; embryonic placenta development; ossification; transcription, DNA-dependent; viral reproduction; megakaryocyte differentiation; positive regulation of transcription, DNA-dependent; embryonic skeletal development; embryonic process involved in female pregnancy; rhythmic process; cellular lipid metabolic process; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; lung development |
| NCBI Summary: | The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
| UniProt Code: | P08047 |
| NCBI GenInfo Identifier: | 13638437 |
| NCBI Gene ID: | 6667 |
| NCBI Accession: | P08047.3 |
| UniProt Secondary Accession: | P08047,Q86TN8, Q9H3Q5, Q9NR51, Q9NY21, Q9NYE7, E4Z9M7 G5E9M8, |
| UniProt Related Accession: | P08047 |
| Molecular Weight: | |
| NCBI Full Name: | Transcription factor Sp1 |
| NCBI Synonym Full Names: | Sp1 transcription factor |
| NCBI Official Symbol: | SP1 |
| NCBI Protein Information: | transcription factor Sp1; specificity protein 1 |
| UniProt Protein Name: | Transcription factor Sp1 |
| Protein Family: | Sex-specific storage-protein |
| UniProt Gene Name: | SP1 |
| UniProt Entry Name: | SP1_HUMAN |
