Description
MYSM1 Transcription Factor Activity Assay
The MySM1 Transcription Factor Activity Assay is a powerful tool for researchers looking to study the activity of the MySM1 transcription factor in cellular pathways. This assay kit provides an accurate and reliable method for detecting and quantifying MySM1 activity in cell lysates, serum, or tissues.MySM1 is a key transcription factor involved in regulating gene expression and is known to play crucial roles in various biological processes, including cell differentiation, proliferation, and apoptosis. Dysregulation of MySM1 activity has been implicated in various diseases, making it a valuable target for drug discovery and therapeutic interventions.
With its high sensitivity and specificity, the MySM1 Transcription Factor Activity Assay offers researchers a robust and convenient tool for studying MySM1 function in both normal and disease states. Whether investigating molecular mechanisms or screening potential drug candidates, this assay kit provides accurate and reproducible results, making it an essential component of any research laboratory.
| Product Name: | MYSM1 Transcription Factor Activity Assay |
| Product Code: | TFAB00189 |
| Target: | MYSM1 |
| Synonyms: | Myb-like, SWIRM and MPN domain-containing protein 1 |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie MYSM1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | MYSM1: Metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Preferentially deubiquitinates monoubiquitinated H2A in hyperacetylated nucleosomes. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. Acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. Belongs to the peptidase M67A family. MYSM1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Ubiquitin-specific protease; EC 3.4.19.-; Protease; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1p32.1 Cellular Component: nucleoplasm; microtubule cytoskeleton; nucleus Molecular Function:DNA binding; metallopeptidase activity; histone binding; metal ion binding; ubiquitin-specific protease activity; transcription coactivator activity; protein complex binding; chromatin binding Biological Process: chromatin remodeling; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; proteolysis |
| UniProt Code: | Q5VVJ2 |
| NCBI GenInfo Identifier: | 74756898 |
| NCBI Gene ID: | 114803 |
| NCBI Accession: | Q5VVJ2.1 |
| UniProt Secondary Accession: | Q5VVJ2,Q68DD3, Q6AI53, Q7Z3G8, Q96PX3, A8KA54, B3KX65 |
| UniProt Related Accession: | Q5VVJ2 |
| Molecular Weight: | 828 |
| NCBI Full Name: | Histone H2A deubiquitinase MYSM1 |
| NCBI Synonym Full Names: | Myb-like, SWIRM and MPN domains 1 |
| NCBI Official Symbol: | MYSM1 |
| NCBI Official Synonym Symbols: | 2ADUB; 2A-DUB |
| NCBI Protein Information: | histone H2A deubiquitinase MYSM1; myb-like, SWIRM and MPN domain-containing protein 1 |
| UniProt Protein Name: | Histone H2A deubiquitinase MYSM1 |
| UniProt Synonym Protein Names: | Myb-like, SWIRM and MPN domain-containing protein 1 |
| Protein Family: | Histone H2A deubiquitinase |
| UniProt Gene Name: | MYSM1 |
| UniProt Entry Name: | MYSM1_HUMAN |
