Description
FLI1 Transcription Factor Activity Assay
The FLI1 Transcription Factor Activity Assay Kit is a powerful tool for measuring the activity of the FLI1 transcription factor in a variety of samples, including cell lysates and tissue homogenates. This assay is highly sensitive and specific, providing accurate and reproducible results for reliable data analysis.The FLI1 transcription factor is known for its key role in regulating gene expression related to cell growth, differentiation, and development. Dysregulation of FLI1 activity has been linked to various diseases, including certain types of cancer and autoimmune disorders.
By accurately measuring FLI1 activity, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the FLI1 Transcription Factor Activity Assay Kit offers a versatile and reliable solution for studying the role of FLI1 in various biological processes and disease pathways. Its user-friendly protocol and high-performance characteristics make it an essential tool for any research laboratory focused on transcription factor regulation and gene expression dynamics.
| Product Name: | FLI1 Transcription Factor Activity Assay |
| Product Code: | TFAB00101 |
| Target: | FLI1 |
| Synonyms: | Proto-oncogene Fli-1, Transcription factor ERGB |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie FLI1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | FLI1: Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. Defects in FLI1 are a cause of Ewing sarcoma (ES). A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 11q24.1-q24.3 Cellular Component: nucleus Molecular Function:DNA binding; chromatin binding; transcription factor activity Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; organ morphogenesis; hemostasis; blood circulation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation |
| NCBI Summary: | This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012] |
| UniProt Code: | Q01543 |
| NCBI GenInfo Identifier: | 399496 |
| NCBI Gene ID: | 2313 |
| NCBI Accession: | Q01543.1 |
| UniProt Secondary Accession: | Q01543,Q14319, Q92480, Q9UE07, B2R8H2, B4DFV4, B4DTC6 G3V183, |
| UniProt Related Accession: | Q01543 |
| Molecular Weight: | |
| NCBI Full Name: | Friend leukemia integration 1 transcription factor |
| NCBI Synonym Full Names: | Fli-1 proto-oncogene, ETS transcription factor |
| NCBI Official Symbol: | FLI1 |
| NCBI Official Synonym Symbols: | EWSR2; SIC-1 |
| NCBI Protein Information: | Friend leukemia integration 1 transcription factor; transcription factor ERGB; Ewing sarcoma breakpoint region 2; Friend leukemia virus integration 1 |
| UniProt Protein Name: | Friend leukemia integration 1 transcription factor |
| UniProt Synonym Protein Names: | Proto-oncogene Fli-1; Transcription factor ERGB |
| Protein Family: | Friend leukemia integration 1 transcription factor |
| UniProt Gene Name: | FLI1 |
| UniProt Entry Name: | FLI1_HUMAN |
