Description
c-Jun (Phospho-Ser249)Transcription Factor Activity Assay
The c-Jun Phospho-Ser249 Transcription Factor Activity Assay offered by AssayGenie is a reliable and efficient tool for the detection and quantification of c-Jun phosphorylation at Ser249, a key site involved in regulating transcription factor activity. This assay provides researchers with high sensitivity and specificity, ensuring accurate and reproducible results in a variety of experimental settings.c-Jun is a critical transcription factor that plays a pivotal role in cellular processes such as proliferation, apoptosis, and differentiation. Phosphorylation of c-Jun at Ser249 is known to modulate its transcriptional activity, making it essential for the regulation of gene expression in response to various stimuli.
By utilizing the c-Jun Phospho-Ser249 Transcription Factor Activity Assay, researchers can gain valuable insights into the molecular mechanisms underlying cellular signaling pathways and gene expression regulation. This assay is suitable for use with a wide range of sample types, including cell lysates and tissue extracts, making it a versatile tool for investigating the role of c-Jun phosphorylation in a variety of biological processes.Overall, the c-Jun Phospho-Ser249 Transcription Factor Activity Assay from AssayGenie is a valuable research tool for studying the intricate workings of transcription factor regulation and its implications in various physiological and pathological conditions.
| Product Name: | c-Jun (Phospho-Ser249)Transcription Factor Activity Assay |
| Product Code: | TFAB00003 |
| Target: | c-Jun (Phospho-Ser249) |
| Synonyms: | Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie c-Jun (Phospho-Ser249) transcription factor activity assay allows for the detection and qualitative analysis of c-Jun (Phospho-Ser249) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). |
| NCBI Summary: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05412 |
| NCBI GenInfo Identifier: | 135298 |
| NCBI Gene ID: | 3725 |
| NCBI Accession: | P05412.2 |
| UniProt Secondary Accession: | P05412,Q6FHM7, Q96G93, |
| UniProt Related Accession: | P05412 |
| Molecular Weight: | |
| NCBI Full Name: | Transcription factor AP-1 |
| NCBI Synonym Full Names: | jun proto-oncogene |
| NCBI Official Symbol: | JUN |
| NCBI Official Synonym Symbols: | AP1; AP-1; c-Jun |
| NCBI Protein Information: | transcription factor AP-1 |
| UniProt Protein Name: | Transcription factor AP-1 |
| UniProt Synonym Protein Names: | Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Protein Family: | C-Jun-amino-terminal kinase-interacting protein |
| UniProt Gene Name: | JUN |
| UniProt Entry Name: | JUN_HUMAN |
