Description
c-Jun (Phospho-Thr93)Transcription Factor Activity Assay
The c-Jun Phospho-Thr93 Transcription Factor Activity Assay kit is a highly sensitive and specific assay that allows for the accurate detection of c-Jun phosphorylation at threonine 93 in cell lysates. This assay is essential for studying the activity of c-Jun, a transcription factor that plays a key role in cellular processes such as proliferation, differentiation, and apoptosis.By measuring the phosphorylation status of c-Jun at threonine 93, researchers can gain valuable insights into the signaling pathways that regulate gene expression and cell behavior. This information is crucial for understanding the role of c-Jun in various biological processes and diseases, including cancer, inflammation, and neurodegeneration.
With its reliable and reproducible results, the c-Jun Phospho-Thr93 Transcription Factor Activity Assay kit is a valuable tool for researchers studying the molecular mechanisms underlying cellular functions and disease progression. Its high sensitivity and specificity make it an ideal choice for a wide range of research applications, helping to advance our understanding of c-Jun biology and its potential as a therapeutic target.
| Product Name: | c-Jun (Phospho-Thr93)Transcription Factor Activity Assay |
| Product Code: | TFAB00009 |
| Target: | c-Jun (Phospho-Thr93) |
| Synonyms: | Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie c-Jun (Phospho-Thr93) transcription factor activity assay allows for the detection and qualitative analysis of c-Jun (Phospho-Thr93) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). |
| NCBI Summary: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05412 |
| NCBI GenInfo Identifier: | 135298 |
| NCBI Gene ID: | 3725 |
| NCBI Accession: | P05412.2 |
| UniProt Secondary Accession: | P05412,Q6FHM7, Q96G93, |
| UniProt Related Accession: | P05412 |
| Molecular Weight: | |
| NCBI Full Name: | Transcription factor AP-1 |
| NCBI Synonym Full Names: | jun proto-oncogene |
| NCBI Official Symbol: | JUN |
| NCBI Official Synonym Symbols: | AP1; AP-1; c-Jun |
| NCBI Protein Information: | transcription factor AP-1 |
| UniProt Protein Name: | Transcription factor AP-1 |
| UniProt Synonym Protein Names: | Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Protein Family: | C-Jun-amino-terminal kinase-interacting protein |
| UniProt Gene Name: | JUN |
| UniProt Entry Name: | JUN_HUMAN |
