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Anti-Phospho-Jun-S63 Antibody (CABP0048)

SKU:
CABP0048
Product Type:
Antibody
Applications:
WB
IF
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-Phospho-Jun-S63 Antibody CABP0048
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-Phospho-Jun-S63 Antibody CABP0048
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Description

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Antibody Name:Anti-Phospho-Jun-S63 Antibody
Antibody SKU:CABP0048
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around S63 of human c-Jun (NP_002219.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:293
Immunogen:A synthetic phosphorylated peptide around S63 of human c-Jun (NP_002219.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LTSP D
Gene ID:3725
Uniprot:P05412
Cellular Location:Nucleus
Calculated MW:35kDa
Observed MW:48kDa
Anti-Phospho-Jun-S63 Antibody (CABP0048)Western blot analysis of extracts of 293 cells, using Phospho-c-Jun-S63 antibody (CABP0048) at 1:1000 dilution. 293 cells were treated by Anisomycin (25ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Anti-Phospho-Jun-S63 Antibody (CABP0048)Immunofluorescence analysis of MCF-7 cells using Phospho-c-Jun-S63 antibody (CABP0048). Blue: DAPI for nuclear staining.
Synonyms:AP-1, AP1, c-Jun, JUN, c
Background:This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
UniProt Protein Function:Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
NCBI Summary:This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
UniProt Code:P05412
NCBI GenInfo Identifier:135298
NCBI Gene ID:3725
NCBI Accession:P05412.2
UniProt Secondary Accession:P05412,Q6FHM7, Q96G93,
UniProt Related Accession:P05412
Molecular Weight:
NCBI Full Name:Transcription factor AP-1
NCBI Synonym Full Names:jun proto-oncogene
NCBI Official Symbol:JUN  
NCBI Official Synonym Symbols:AP1; AP-1; c-Jun  
NCBI Protein Information:transcription factor AP-1
UniProt Protein Name:Transcription factor AP-1
UniProt Synonym Protein Names:Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
Protein Family:C-Jun-amino-terminal kinase-interacting protein
UniProt Gene Name:JUN  
UniProt Entry Name:JUN_HUMAN

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