Description
c-Jun (Phospho-Ser63)Transcription Factor Activity Assay
The c-Jun Phospho-Ser63 Transcription Factor Activity Assay Kit is a highly sensitive and specific assay designed to measure the activity of the transcription factor c-Jun when phosphorylated at serine 63. This kit is suitable for the accurate detection of c-Jun activity in various sample types, including cell lysates and tissue homogenates.c-Jun is a key transcription factor that plays a crucial role in regulating gene expression, cell proliferation, and cell survival. Phosphorylation of c-Jun at serine 63 is known to enhance its transcriptional activity, making it essential for cell growth and differentiation processes.
Dysregulation of c-Jun has been implicated in various diseases, including cancer and inflammatory disorders, highlighting its importance as a potential therapeutic target.With its high sensitivity and reproducibility, the c-Jun Phospho-Ser63 Transcription Factor Activity Assay Kit is an invaluable tool for researchers studying the molecular mechanisms underlying c-Jun signaling and its role in disease pathogenesis. Its user-friendly protocol and reliable results make it ideal for use in a wide range of research applications.
| Product Name: | c-Jun (Phospho-Ser63)Transcription Factor Activity Assay |
| Product Code: | TFAB00004 |
| Target: | c-Jun (Phospho-Ser63) |
| Synonyms: | Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie c-Jun (Phospho-Ser63) transcription factor activity assay allows for the detection and qualitative analysis of c-Jun (Phospho-Ser63) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). |
| NCBI Summary: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P05412 |
| NCBI GenInfo Identifier: | 135298 |
| NCBI Gene ID: | 3725 |
| NCBI Accession: | P05412.2 |
| UniProt Secondary Accession: | P05412,Q6FHM7, Q96G93, |
| UniProt Related Accession: | P05412 |
| Molecular Weight: | |
| NCBI Full Name: | Transcription factor AP-1 |
| NCBI Synonym Full Names: | jun proto-oncogene |
| NCBI Official Symbol: | JUN |
| NCBI Official Synonym Symbols: | AP1; AP-1; c-Jun |
| NCBI Protein Information: | transcription factor AP-1 |
| UniProt Protein Name: | Transcription factor AP-1 |
| UniProt Synonym Protein Names: | Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Protein Family: | C-Jun-amino-terminal kinase-interacting protein |
| UniProt Gene Name: | JUN |
| UniProt Entry Name: | JUN_HUMAN |
