Smad3 Transcription Factor Activity Assay



ELISA Kit Technical Manual

Smad3 Transcription Factor activity assay

Product Description:The Assay Genie Smad3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates Assay Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:JV15-2;SMAD family member 3 Short name: SMAD 3 Short name: Smad3 Short name: hSMAD3
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse, Rat
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

Smad3 Transcription Factor activity assay Protein Information

UniProt Protein Function:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
NCBI Summary:The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
UniProt Code:P84022
NCBI GenInfo Identifier:51338669
NCBI Gene ID:4088
NCBI Accession:P84022.1
UniProt Secondary Accession:P84022,O09064, O09144, O14510, O35273, Q92940, Q93002 A8K4B6, B7Z4Z5, B7Z6M9, B7Z9Q2, F5H383,
UniProt Related Accession:P84022
Molecular Weight:25,722 Da
NCBI Full Name:Mothers against decapentaplegic homolog 3
NCBI Synonym Full Names:SMAD family member 3
NCBI Official Symbol:SMAD3
NCBI Official Synonym Symbols:LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
NCBI Protein Information:mothers against decapentaplegic homolog 3
UniProt Protein Name:Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names:JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family:Mothers against decapentaplegic
UniProt Gene Name:SMAD3
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Additional Information

Product Type:
Transcription Factor Assay
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