null

Anti-Smad3 Antibody [KO Validated] (CAB19115)

SKU:
CAB19115
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • KO Validated Antibodies 2 Anti-Smad3 Antibody KO Validated CAB19115
  • KO Validated Antibodies 2 Anti-Smad3 Antibody KO Validated CAB19115
  • KO Validated Antibodies 2 Anti-Smad3 Antibody KO Validated CAB19115
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Smad3 Antibody [KO Validated]
Antibody SKU:CAB19115
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Smad3
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:A-431, Mouse brain, Rat brain, 293T
Immunogen:A synthesized peptide derived from human Smad3
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4088
Uniprot:P84022
Cellular Location:Cytoplasm, Nucleus
Calculated MW:58kDa
Observed MW:58kDa
Anti-Smad3 Antibody [KO Validated] (CAB19115)Western blot analysis of extracts from normal (control) and Smad3 knockout (KO) 293T cells, using Smad3 antibody (CAB19115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Smad3 Antibody [KO Validated] (CAB19115)Western blot analysis of extracts of various cell lines, using Smad3 antibody (CAB19115) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-Smad3 Antibody [KO Validated] (CAB19115)Immunohistochemistry of paraffin-embedded human liver using Smad3 Rabbit mAb (CAB19115) at dilution of 1:100 (40x lens).
Synonyms:HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, SMAD3
Background:The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
UniProt Protein Function:Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
NCBI Summary:The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
UniProt Code:P84022
NCBI GenInfo Identifier:51338669
NCBI Gene ID:4088
NCBI Accession:P84022.1
UniProt Secondary Accession:P84022,O09064, O09144, O14510, O35273, Q92940, Q93002 A8K4B6, B7Z4Z5, B7Z6M9, B7Z9Q2, F5H383,
UniProt Related Accession:P84022
Molecular Weight:25,722 Da
NCBI Full Name:Mothers against decapentaplegic homolog 3
NCBI Synonym Full Names:SMAD family member 3
NCBI Official Symbol:SMAD3  
NCBI Official Synonym Symbols:LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436  
NCBI Protein Information:mothers against decapentaplegic homolog 3
UniProt Protein Name:Mothers against decapentaplegic homolog 3
UniProt Synonym Protein Names:JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Protein Family:Mothers against decapentaplegic
UniProt Gene Name:SMAD3  

Related Products