TTF1 Transcription Factor Activity Assay



ELISA Kit Technical Manual

TTF1 Transcription Factor activity assay

Product Description:The Assay Genie TTF1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates Assay Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:
Detection Target:Phospho-IRAK1(Thr209)
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

TTF1 Transcription Factor activity assay Protein Information

UniProt Protein Function:TTF1: Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity.
UniProt Protein Details:

Protein type:Nucleolus; DNA-binding

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; plasma membrane

Molecular Function:chromatin binding; DNA binding

Biological Process: cellular protein metabolic process; chromatin remodeling; gene expression; negative regulation of DNA replication; negative regulation of gene expression, epigenetic; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription initiation from RNA polymerase I promoter; transcription termination

NCBI Summary:This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011]
UniProt Code:Q15361
NCBI GenInfo Identifier:328927005
NCBI Gene ID:7270
NCBI Accession:NP_001192225.1
UniProt Secondary Accession:Q15361,Q4VXF3, Q58EY2, Q6P5T5, A1L160,
UniProt Related Accession:Q15361
Molecular Weight:103,051 Da
NCBI Full Name:transcription termination factor 1 isoform 2
NCBI Synonym Full Names:transcription termination factor, RNA polymerase I
NCBI Official Symbol:TTF1
NCBI Official Synonym Symbols:TTF-1; TTF-I
NCBI Protein Information:transcription termination factor 1
UniProt Protein Name:Transcription termination factor 1
UniProt Synonym Protein Names:RNA polymerase I termination factor; Transcription termination factor I; TTF-I
Protein Family:Transcription termination factor
UniProt Gene Name:TTF1
UniProt Entry Name:TTF1_HUMAN
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Additional Information

Product Type:
Transcription Factor Assay
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