Description
TTF1 Transcription Factor Activity Assay
The TTF1 Transcription Factor Activity Assay is a versatile and reliable kit for the assessment of TTF1 transcription factor activity in various sample types including cell lysates and tissue homogenates. This assay provides high sensitivity and specificity, allowing for accurate and reproducible results in a wide range of research applications.TTF1, also known as Thyroid Transcription Factor 1, plays a crucial role in the regulation of gene expression related to lung development and function.
Dysregulation of TTF1 has been implicated in various respiratory diseases including lung cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With the TTF1 Transcription Factor Activity Assay, researchers can confidently measure TTF1 activity and gain insight into its function in disease pathogenesis, paving the way for advancements in the diagnosis and treatment of lung-related disorders.
| Product Name: | TTF1 Transcription Factor Activity Assay |
| Product Code: | TFAB00191 |
| Target: | TTF1 |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie TTF1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | TTF1: Multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. Plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5 recovers DNA-binding activity. |
| UniProt Protein Details: | Protein type:Nucleolus; DNA-binding Chromosomal Location of Human Ortholog: 9q34.13 Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; plasma membrane Molecular Function:chromatin binding; DNA binding Biological Process: cellular protein metabolic process; chromatin remodeling; gene expression; negative regulation of DNA replication; negative regulation of gene expression, epigenetic; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; termination of RNA polymerase I transcription; transcription from RNA polymerase I promoter; transcription initiation from RNA polymerase I promoter; transcription termination |
| NCBI Summary: | This gene encodes a transcription termination factor that is localized to the nucleolus and plays a critical role in ribosomal gene transcription. The encoded protein mediates the termination of RNA polymerase I transcription by binding to Sal box terminator elements downstream of pre-rRNA coding regions. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene shares the symbol/alias 'TFF1' with another gene, NK2 homeobox 1, also known as thyroid transcription factor 1, which plays a role in the regulation of thyroid-specific gene expression. [provided by RefSeq, Apr 2011] |
| UniProt Code: | Q15361 |
| NCBI GenInfo Identifier: | 328927005 |
| NCBI Gene ID: | 7270 |
| NCBI Accession: | NP_001192225.1 |
| UniProt Secondary Accession: | Q15361,Q4VXF3, Q58EY2, Q6P5T5, A1L160, |
| UniProt Related Accession: | Q15361 |
| Molecular Weight: | 103,051 Da |
| NCBI Full Name: | transcription termination factor 1 isoform 2 |
| NCBI Synonym Full Names: | transcription termination factor, RNA polymerase I |
| NCBI Official Symbol: | TTF1 |
| NCBI Official Synonym Symbols: | TTF-1; TTF-I |
| NCBI Protein Information: | transcription termination factor 1 |
| UniProt Protein Name: | Transcription termination factor 1 |
| UniProt Synonym Protein Names: | RNA polymerase I termination factor; Transcription termination factor I; TTF-I |
| Protein Family: | Transcription termination factor |
| UniProt Gene Name: | TTF1 |
| UniProt Entry Name: | TTF1_HUMAN |
