Description
IRF2 Transcription Factor Activity Assay
The IRF2 Transcription Factor Activity Assay Kit is specifically designed for the precise measurement of IRF2 transcription factor activity in various biological samples. This kit offers high sensitivity and accuracy, allowing researchers to obtain reliable and consistent results for their experiments.IRF2 is a key transcription factor that plays a critical role in regulating gene expression, particularly in immune responses and cell differentiation. Dysregulation of IRF2 activity has been linked to various diseases, including cancer, autoimmune disorders, and inflammatory conditions, highlighting its importance as a potential therapeutic target.
With the IRF2 Transcription Factor Activity Assay Kit, researchers can explore the intricate mechanisms underlying IRF2 function and its implications in disease pathology. This easy-to-use kit is suitable for a wide range of research applications, providing valuable insights into the role of IRF2 in health and disease.
| Product Name: | IRF2 Transcription Factor Activity Assay |
| Product Code: | TFAB00131 |
| Target: | IRF2 |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie IRF2 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | IRF2: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity. By viruses and IFN. Expressed throughout the epithelium of the colon. Also expressed in lamina propria. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 4q34.1-q35.1 Cellular Component: nucleoplasm; focal adhesion; cytoplasm; cytosol Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; blood coagulation |
| NCBI Summary: | IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14316 |
| NCBI GenInfo Identifier: | 20141499 |
| NCBI Gene ID: | 3660 |
| NCBI Accession: | P14316.2 |
| UniProt Secondary Accession: | P14316,Q6IAS7, Q96B99, D6RCK5, H0Y8S3, |
| UniProt Related Accession: | P14316 |
| Molecular Weight: | 39,156 Da |
| NCBI Full Name: | Interferon regulatory factor 2 |
| NCBI Synonym Full Names: | interferon regulatory factor 2 |
| NCBI Official Symbol: | IRF2 |
| NCBI Official Synonym Symbols: | IRF-2 |
| NCBI Protein Information: | interferon regulatory factor 2 |
| UniProt Protein Name: | Interferon regulatory factor 2 |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | IRF2 |
| UniProt Entry Name: | IRF2_HUMAN |
