Description
ATF5 Transcription Factor Activity Assay
The ATF5 Transcription Factor Activity Assay is a powerful tool for researchers studying transcriptional regulation and gene expression. This assay kit allows for the accurate measurement of ATF5 activity in cell lysates or nuclear extracts, providing valuable insights into the role of ATF5 in various cellular processes.ATF5 is a transcription factor that plays a key role in regulating gene expression in response to cellular stress, making it a critical mediator of cell survival and proliferation. Dysregulation of ATF5 has been implicated in a variety of diseases, including cancer and neurodegenerative disorders, highlighting its importance as a potential therapeutic target.
The ATF5 Transcription Factor Activity Assay offers high sensitivity and specificity, ensuring precise and reliable results that can be easily quantified using a microplate reader. By tracking ATF5 activity, researchers can gain a better understanding of its impact on gene expression and potentially identify novel treatment strategies for diseases associated with ATF5 dysregulation.
| Product Name: | ATF5 Transcription Factor Activity Assay |
| Product Code: | TFAB00085 |
| Target: | ATF5 |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ATF5 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ATF-5: Transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Belongs to the bZIP family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: centrosome; cytoplasm; nucleoplasm; nucleus Molecular Function:chromatin binding; kinase binding; protein binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity; tubulin binding Biological Process: fat cell differentiation; granule cell precursor proliferation; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of centrosome cycle; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent |
| UniProt Code: | Q9Y2D1 |
| NCBI GenInfo Identifier: | 308153647 |
| NCBI Gene ID: | 22809 |
| NCBI Accession: | Q9Y2D1.4 |
| UniProt Secondary Accession: | Q9Y2D1,Q9BSA1, Q9UNQ3, B3KND3, |
| UniProt Related Accession: | Q9Y2D1 |
| Molecular Weight: | 30,674 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-5 |
| NCBI Synonym Full Names: | activating transcription factor 5 |
| NCBI Official Symbol: | ATF5 |
| NCBI Official Synonym Symbols: | ATFX; HMFN0395 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-5 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-5 |
| UniProt Synonym Protein Names: | Activating transcription factor 5; Transcription factor ATFx |
| Protein Family: | Cyclic AMP-dependent transcription factor |
| UniProt Gene Name: | ATF5 |
| UniProt Entry Name: | ATF5_HUMAN |
