Description
ATF2 (Phospho-Ser480)Transcription Factor Activity Assay
The ATF2 Phospho-Ser480 Transcription Factor Activity Assay is a highly sensitive and specific assay kit designed to accurately detect ATF2 phosphorylation at serine 480 in cell lysates. This assay is essential for studying the transcription factor activity of ATF2, a key regulator of gene expression involved in various cellular processes.ATF2 plays a critical role in the cellular response to stress, DNA damage, and inflammatory stimuli, making it a crucial player in the development and progression of diseases such as cancer, neurodegenerative disorders, and inflammatory conditions.
By measuring ATF2 phosphorylation at serine 480, researchers can gain valuable insights into the transcriptional activity of ATF2 and its role in disease pathogenesis.With its high sensitivity, specificity, and reproducibility, the ATF2 Phospho-Ser480 Transcription Factor Activity Assay is an indispensable tool for researchers looking to investigate the role of ATF2 in disease mechanisms and identify potential therapeutic targets.
| Product Name: | ATF2 (Phospho-Ser480)Transcription Factor Activity Assay |
| Product Code: | TFAB00014 |
| Target: | ATF2 (Phospho-Ser480) |
| Synonyms: | Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, HB16, Histone acetyltransferase ATF2, cAMP response element-binding protein CRE-BP1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ATF2 (Phospho-Ser480) transcription factor activity assay allows for the detection and qualitative analysis of ATF2 (Phospho-Ser480) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro. |
| UniProt Protein Details: | Protein type:EC 2.3.1.48; Transcription factor; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 2q32 Cellular Component: nucleoplasm; mitochondrial outer membrane; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; histone acetyltransferase activity; protein heterodimerization activity; metal ion binding; cAMP response element binding protein binding; transcription coactivator activity; chromatin binding; transcription factor activity; protein kinase binding Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; positive regulation of transforming growth factor-beta2 production; response to osmotic stress; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transcription factor activity; intra-S DNA damage checkpoint; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; histone acetylation; toll-like receptor 9 signaling pathway; response to DNA damage stimulus; toll-like receptor 4 signaling pathway |
| NCBI Summary: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014] |
| UniProt Code: | P15336 |
| NCBI GenInfo Identifier: | 215274241 |
| NCBI Gene ID: | 1386 |
| NCBI Accession: | P15336.4 |
| UniProt Secondary Accession: | P15336,Q13000, Q3B7B7, Q4ZFU9, Q53RY2, Q8TAR1, A1L3Z2 A4D7U4, A4D7U5, A4D7V1, D3DPE9, G8JLM5, |
| UniProt Related Accession: | P15336 |
| Molecular Weight: | 505 |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-2 |
| NCBI Synonym Full Names: | activating transcription factor 2 |
| NCBI Official Symbol: | ATF2 |
| NCBI Official Synonym Symbols: | HB16; CREB2; TREB7; CREB-2; CRE-BP1 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-2; histone acetyltransferase ATF2; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; cAMP response element-binding protein CRE-BP1; cyclic AMP-responsive element-binding protein 2; cAMP responsive element binding protein 2, formerly; activating transcription factor 2 splice variant ATF2-var2 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-2 |
| UniProt Synonym Protein Names: | Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1 |
| Protein Family: | Alcohol O-acetyltransferase |
| UniProt Gene Name: | ATF2 |
| UniProt Entry Name: | ATF2_HUMAN |
