ATF2 (Phospho-Thr71 or 53) Transcription Factor Activity Assay

Product Name:	ATF2 (Phospho-Thr71 or 53) Transcription Factor Activity Assay
Product Code:	TFAB00016
Target:	ATF2 (Phospho-Thr71 or 53)
Synonyms:	Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, CREB-2, cAMP-responsive element-binding protein 2, HB16, Histone acetyltransferase ATF2, cAMP response element-binding protein CRE-BP1
Reactivity:	Human, Mouse, Rat
Sample Types:	Nuclear or cell lysates

The Assay Genie ATF2 (Phospho-Thr71 or 53) transcription factor activity assay allows for the detection and qualitative analysis of ATF2 (Phospho-Thr71 or 53) active transcription factors in eukaryotic nuclear or cell lysates.

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:	4.5 hours
Detection Method:	Colorimetric 450 nm
Size:	12 x 8-Well Microstrips
Storage:	4°C for 6 months

UniProt Protein Function:	ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.
UniProt Protein Details:	Protein type:EC 2.3.1.48; Transcription factor; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 2q32 Cellular Component: nucleoplasm; mitochondrial outer membrane; cytoplasm; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; histone acetyltransferase activity; protein heterodimerization activity; metal ion binding; cAMP response element binding protein binding; transcription coactivator activity; chromatin binding; transcription factor activity; protein kinase binding Biological Process: fat cell differentiation; transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; positive regulation of transforming growth factor-beta2 production; response to osmotic stress; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transcription factor activity; intra-S DNA damage checkpoint; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; histone acetylation; toll-like receptor 9 signaling pathway; response to DNA damage stimulus; toll-like receptor 4 signaling pathway
NCBI Summary:	This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]
UniProt Code:	P15336
NCBI GenInfo Identifier:	215274241
NCBI Gene ID:	1386
NCBI Accession:	P15336.4
UniProt Secondary Accession:	P15336,Q13000, Q3B7B7, Q4ZFU9, Q53RY2, Q8TAR1, A1L3Z2 A4D7U4, A4D7U5, A4D7V1, D3DPE9, G8JLM5,
UniProt Related Accession:	P15336
Molecular Weight:	505
NCBI Full Name:	Cyclic AMP-dependent transcription factor ATF-2
NCBI Synonym Full Names:	activating transcription factor 2
NCBI Official Symbol:	ATF2
NCBI Official Synonym Symbols:	HB16; CREB2; TREB7; CREB-2; CRE-BP1
NCBI Protein Information:	cyclic AMP-dependent transcription factor ATF-2; histone acetyltransferase ATF2; cAMP-dependent transcription factor ATF-2; cAMP-responsive element-binding protein 2; cAMP response element-binding protein CRE-BP1; cyclic AMP-responsive element-binding protein 2; cAMP responsive element binding protein 2, formerly; activating transcription factor 2 splice variant ATF2-var2
UniProt Protein Name:	Cyclic AMP-dependent transcription factor ATF-2
UniProt Synonym Protein Names:	Activating transcription factor 2; Cyclic AMP-responsive element-binding protein 2; CREB-2; cAMP-responsive element-binding protein 2; HB16; Histone acetyltransferase ATF2; cAMP response element-binding protein CRE-BP1
Protein Family:	Alcohol O-acetyltransferase
UniProt Gene Name:	ATF2
UniProt Entry Name:	ATF2_HUMAN

Antibodies	ELISA Kits
Anti-ATF2 Antibody (CAB2155)[KO Validated]	Human ATF2 (Activating Transcription Factor 2) ELISA Kit (HUES01718)
Anti-Phospho-ATF2-T71 Antibody (CABP0020)	ATF2 (Phospho-Ser480) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-ATF2-T69/T71 Antibody (CABP0525)	ATF2 (Phospho-Ser112) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-ATF2-T69/71 Antibody (CABP0913)	ATF2 (Phospho-Thr691) Colorimetric Cell-Based ELISA Kit
Anti-Phospho-ATF2-T71 Antibody (CABP1051)	ATF2 (Phospho-Thr71) Colorimetric Cell-Based ELISA Kit