Description
NFYA Transcription Factor Activity Assay
The NFYA Transcription Factor Activity Assay is a powerful tool designed for the detection and quantification of NFYA activity in various biological samples. NFYA is a key transcription factor that plays a crucial role in regulating gene expression and controlling a wide range of cellular processes.This assay kit offers high sensitivity and specificity, allowing for accurate and reliable measurement of NFYA activity in cell lysates, tissue homogenates, and other sample types. By accurately measuring NFYA activity, researchers can gain valuable insights into the mechanisms underlying gene regulation, cell differentiation, and disease development.
The NFYA Transcription Factor Activity Assay is a valuable resource for scientists studying transcriptional regulation, gene expression, and the role of NFYA in various biological processes. With its user-friendly protocols and robust performance, this assay kit is suitable for a wide range of research applications, from basic science to drug discovery and therapeutic development. Unlock the potential of NFYA research with the NFYA Transcription Factor Activity Assay.
| Product Name: | NFYA Transcription Factor Activity Assay |
| Product Code: | TFAB00142 |
| Target: | NFYA |
| Synonyms: | CAAT box transcription factor activity protein subunit A, Nuclear transcription factor Y subunit A, NF-YA |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie NFYA transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | NFYA: stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. Involved in cisplatin resistance. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 6p21.1 Cellular Component: CCAAT-binding factor complex; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter |
| NCBI Summary: | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P23511 |
| NCBI GenInfo Identifier: | 115844 |
| NCBI Gene ID: | 4800 |
| NCBI Accession: | P23511.2 |
| UniProt Secondary Accession: | P23511,Q8IXU0, |
| UniProt Related Accession: | P23511 |
| Molecular Weight: | 37kDa |
| NCBI Full Name: | Nuclear transcription factor Y subunit alpha |
| NCBI Synonym Full Names: | nuclear transcription factor Y subunit alpha |
| NCBI Official Symbol: | NFYA |
| NCBI Official Synonym Symbols: | HAP2; CBF-A; CBF-B; NF-YA |
| NCBI Protein Information: | nuclear transcription factor Y subunit alpha |
| UniProt Protein Name: | Nuclear transcription factor Y subunit alpha |
| UniProt Synonym Protein Names: | CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; NF-YA |
| Protein Family: | Nuclear transcription factor |
| UniProt Gene Name: | NFYA |
