Description
FOXH1 Transcription Factor Activity Assay
The FOXH1 Transcription Factor Activity Assay provided by Assay Genie is a robust and highly sensitive kit designed for the detection and quantification of FOXH1 transcription factor activity in various samples. This assay kit offers researchers a reliable and accurate method for studying the role of FOXH1 in gene regulation and its involvement in important cellular processes.FOXH1 is a key transcription factor that plays a critical role in embryonic development, specifically in the regulation of genes involved in cell differentiation and patterning.
Dysregulation of FOXH1 has been linked to various diseases and disorders, making it an essential target for understanding disease mechanisms and developing potential therapeutic interventions.With its exceptional performance and ease of use, the FOXH1 Transcription Factor Activity Assay from Assay Genie is an invaluable tool for researchers in the fields of developmental biology, molecular biology, and genetics. Gain valuable insights into FOXH1 function and advance your research with confidence using this cutting-edge assay kit.
| Product Name: | FOXH1 Transcription Factor Activity Assay |
| Product Code: | TFAB00110 |
| Target: | FOXH1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie FOXH1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | FOXH1: Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). |
| UniProt Protein Details: | Protein type:DNA-binding; Nuclear receptor co-regulator; Transcription factor Chromosomal Location of Human Ortholog: 8q24.3 Cellular Component: nuclear chromatin; nucleoplasm; nucleus; transcription factor complex Molecular Function:androgen receptor binding; bHLH transcription factor binding; DNA binding; protein binding; protein domain specific binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; SMAD binding; transcription factor activity Biological Process: anatomical structure morphogenesis; cell differentiation; negative regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway Disease: Holoprosencephaly 1 |
| NCBI Summary: | FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O75593 |
| NCBI GenInfo Identifier: | 4503657 |
| NCBI Gene ID: | 8928 |
| NCBI Accession: | NP_003914.1 |
| UniProt Secondary Accession: | O75593,D3DWM4, |
| UniProt Related Accession: | O75593 |
| Molecular Weight: | 39,257 Da |
| NCBI Full Name: | forkhead box protein H1 |
| NCBI Synonym Full Names: | forkhead box H1 |
| NCBI Official Symbol: | FOXH1 |
| NCBI Official Synonym Symbols: | FAST1; FAST-1 |
| NCBI Protein Information: | forkhead box protein H1 |
| UniProt Protein Name: | Forkhead box protein H1 |
| UniProt Synonym Protein Names: | Forkhead activin signal transducer 1; Fast-1; hFAST-1; Forkhead activin signal transducer 2; Fast-2 |
| Protein Family: | FAST kinase domain-containing protein |
| UniProt Gene Name: | FOXH1 |
