Description
DLX5 Transcription Factor Activity Assay
The DLX5 Transcription Factor Activity Assay is a cutting-edge tool for studying the activity of the DLX5 transcription factor in various biological samples. This assay kit offers high sensitivity and precision, allowing researchers to accurately measure DLX5 activity in cell lysates, tissue homogenates, and other sample types.DLX5 is a key transcription factor that plays a critical role in the development and differentiation of various cell types, including bone, muscle, and neural cells. Dysregulation of DLX5 activity has been linked to several diseases and disorders, making it a valuable target for research and therapeutic intervention.
By utilizing the DLX5 Transcription Factor Activity Assay, researchers can gain valuable insights into the molecular mechanisms controlled by DLX5 and its potential implications in disease pathology. This kit provides a reliable and efficient way to assess DLX5 activity, making it an essential tool for studies in developmental biology, regenerative medicine, and disease modeling.
| Product Name: | DLX5 Transcription Factor Activity Assay |
| Product Code: | TFAB00090 |
| Target: | DLX5 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie DLX5 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | DLX5: Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding. Defects in DLX5 are the cause of split-hand/foot malformation type 1, with sensorineural hearing loss (SHFM1D). A disease characterized by the association of split- hand/foot malformation with deafness. Split-hand/foot malformation is a limb malformation involving the central rays of the autopod and presenting with syndactyly, median clefts of the hands and feet, and aplasia and/or hypoplasia of the phalanges, metacarpals, and metatarsals. Some patients have been found to have mental retardation, ectodermal and craniofacial findings, and orofacial clefting. Belongs to the distal-less homeobox family. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 7q22 Cellular Component: nuclear chromatin Biological Process: BMP signaling pathway; cell proliferation; endochondral ossification; nervous system development; osteoblast differentiation; positive regulation of transcription, DNA-dependent; skeletal development Disease: Split-hand/foot Malformation 1 With Sensorineural Hearing Loss, Autosomal Recessive |
| NCBI Summary: | This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein may play a role in bone development and fracture healing. Mutation in this gene, which is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 7, may be associated with split-hand/split-foot malformation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P56178 |
| NCBI GenInfo Identifier: | 12644329 |
| NCBI Gene ID: | 1749 |
| NCBI Accession: | P56178.2 |
| UniProt Secondary Accession: | P56178,Q9UPL1, B7Z4P3, |
| UniProt Related Accession: | P56178 |
| Molecular Weight: | 20,900 Da |
| NCBI Full Name: | Homeobox protein DLX-5 |
| NCBI Synonym Full Names: | distal-less homeobox 5 |
| NCBI Official Symbol: | DLX5 |
| NCBI Official Synonym Symbols: | SHFM1D |
| NCBI Protein Information: | homeobox protein DLX-5 |
| UniProt Protein Name: | Homeobox protein DLX-5 |
| Protein Family: | Homeobox protein |
| UniProt Gene Name: | DLX5 |
| UniProt Entry Name: | DLX5_HUMAN |
