Description
GR Transcription Factor Activity Assay
The GR (Glucocorticoid Receptor) Transcription Factor Activity Assay Kit is a powerful tool for measuring the activity of the GR protein in cell lysates. This kit provides researchers with a reliable and high-throughput method for studying the transcriptional activity of the GR protein, offering valuable insights into gene regulation and cellular responses to glucocorticoid hormones.The GR protein is a key regulator of gene expression, playing a critical role in the body's response to stress and inflammation. Dysregulation of GR activity has been linked to various diseases, including autoimmune disorders, metabolic conditions, and neurological disorders.
By accurately measuring GR transcriptional activity, researchers can gain a better understanding of these diseases and potentially identify new therapeutic targets.This assay kit offers high sensitivity and specificity, ensuring precise and reproducible results. It is easy to use and can be adapted for use in various experimental settings, making it an indispensable tool for researchers studying the biology of the GR protein. With the GR Transcription Factor Activity Assay Kit, researchers can advance their understanding of gene regulation and uncover new insights into the role of the GR protein in health and disease.
| Product Name: | GR Transcription Factor Activity Assay |
| Product Code: | TFAB00118 |
| Target: | GR |
| Synonyms: | Nuclear receptor subfamily 3 group C member 1 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie GR transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Mitochondrial; DNA-binding; Transcription factor; Nuclear receptor Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm; cytosol; membrane; mitochondrial matrix; nucleoplasm; nucleus Molecular Function:glucocorticoid receptor activity; protein binding; protein dimerization activity; steroid binding; transcription factor activity; zinc ion binding Biological Process: adrenal gland development; chromatin modification; gene expression; glucocorticoid mediated signaling; glucocorticoid metabolic process; glucocorticoid receptor signaling pathway; maternal behavior; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; regulation of glucocorticoid biosynthetic process; regulation of gluconeogenesis; regulation of transcription, DNA-dependent; signal transduction; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent Disease: Glucocorticoid Resistance, Generalized |
| NCBI Summary: | This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011] |
| UniProt Code: | P04150 |
| NCBI GenInfo Identifier: | 121069 |
| NCBI Gene ID: | 2908 |
| NCBI Accession: | P04150.1 |
| UniProt Secondary Accession: | P04150,P04151, Q53EP5, Q6N0A4, A0ZXF9, B0LPG8, D3DQF4 |
| UniProt Related Accession: | P04150 |
| Molecular Weight: | 82,904 Da |
| NCBI Full Name: | Glucocorticoid receptor |
| NCBI Synonym Full Names: | nuclear receptor subfamily 3 group C member 1 |
| NCBI Official Symbol: | NR3C1 |
| NCBI Official Synonym Symbols: | GR; GCR; GRL; GCCR; GCRST |
| NCBI Protein Information: | glucocorticoid receptor |
| UniProt Protein Name: | Glucocorticoid receptor |
| UniProt Synonym Protein Names: | Nuclear receptor subfamily 3 group C member 1 |
| Protein Family: | Glucocorticoid receptor |
| UniProt Gene Name: | NR3C1 |
| UniProt Entry Name: | GCR_HUMAN |
