Anti-NR3C1 Antibody (CAB2164)

Antibody Name:	Anti-NR3C1 Antibody
Antibody SKU:	CAB2164
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NR3C1 (NP_001191194.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human NR3C1 (NP_001191194.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSK ESLT PGRE ENPS SVLA QERG DVMD FYKT LRGG ATVK VSAS SPSL AVAS QSDS KQRR LLVD FPKG SVSN AQQP DLSK AVSL SMGL YMGE TETK VMGN DLGF PQQG QISL SSGE TDLK LLEE SIAN LNRS TSVP ENPK SSAS TAVS AAPT EKEF PKTH SDVS SEQQ HLKG QTGT NGGN VKLY TTDQ STFD ILQD LEFS SGSP GKET NESP WRSD LLID ENCL LSPL AGED DSFL LEGN SNED CKPL ILPD TKPK IKDN GDLV LSSP SNVT LPQV KTEK
Gene ID:	2908
Uniprot:	P04150
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, Nucleus
Calculated MW:	49-85kDa
Observed MW:	105kDa

Synonyms:

GCCR, GCR, GCRST, GR, GRL, NR3C1

Background:

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).

UniProt Protein Function:	GR: transcription factor of the nuclear receptor family. Receptor for glucocorticoids (GC). Binds to glucocorticoid response elements (GRE) and modulates other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Mitochondrial; DNA-binding; Transcription factor; Nuclear receptor Chromosomal Location of Human Ortholog: 5q31.3 Cellular Component: cytoplasm; cytosol; membrane; mitochondrial matrix; nucleoplasm; nucleus Molecular Function:glucocorticoid receptor activity; protein binding; protein dimerization activity; steroid binding; transcription factor activity; zinc ion binding Biological Process: adrenal gland development; chromatin modification; gene expression; glucocorticoid mediated signaling; glucocorticoid metabolic process; glucocorticoid receptor signaling pathway; maternal behavior; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; regulation of glucocorticoid biosynthetic process; regulation of gluconeogenesis; regulation of transcription, DNA-dependent; signal transduction; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent Disease: Glucocorticoid Resistance, Generalized
NCBI Summary:	This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]
UniProt Code:	P04150
NCBI GenInfo Identifier:	121069
NCBI Gene ID:	2908
NCBI Accession:	P04150.1
UniProt Secondary Accession:	P04150,P04151, Q53EP5, Q6N0A4, A0ZXF9, B0LPG8, D3DQF4
UniProt Related Accession:	P04150
Molecular Weight:	82,904 Da
NCBI Full Name:	Glucocorticoid receptor
NCBI Synonym Full Names:	nuclear receptor subfamily 3 group C member 1
NCBI Official Symbol:	NR3C1
NCBI Official Synonym Symbols:	GR; GCR; GRL; GCCR; GCRST
NCBI Protein Information:	glucocorticoid receptor
UniProt Protein Name:	Glucocorticoid receptor
UniProt Synonym Protein Names:	Nuclear receptor subfamily 3 group C member 1
Protein Family:	Glucocorticoid receptor
UniProt Gene Name:	NR3C1
UniProt Entry Name:	GCR_HUMAN

	Western blot analysis of extracts of various cell lines, using NR3C1 antibody (CAB2164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat spleen using NR3C1 Rabbit pAb (CAB2164) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver cancer using NR3C1 Rabbit pAb (CAB2164) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-NR3C1 Antibody (CAB13667)	Human NR3C1 / Glucocorticoid Receptor ELISA Kit
Anti-Phospho-NR3C1-S211 pAb Antibody (CABP0759)	Human GR alpha (Glucocorticoid Receptor Alpha) CLIA Kit (HUES01082)
Anti-Glucocorticoid Receptor Antibody (CAB19583)	Human Glucocorticoid receptor (NR3C1) ELISA Kit
NR3C1 Antibody (PACO00094)	GR (Phospho-Ser211) Transcription Factor Activity Assay
Phospho-NR3C1 (S211) Antibody (PACO00304)	GR Transcription Factor Activity Assay

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)