Description
NR1I3 Transcription Factor Activity Assay
The NR1I3 Transcription Factor Activity Assay kit is specifically designed for the precise measurement of NR1I3 transcription factor activity in various biological samples. This assay kit offers high specificity and sensitivity, ensuring accurate and reproducible results for researchers studying the role of NR1I3 in gene regulation and expression.NR1I3, also known as the Pregnane X Receptor (PXR), is a key transcription factor involved in the regulation of drug metabolism and detoxification pathways. Its activity is crucial in determining the response of cells to xenobiotics and various drugs, making it a valuable target for understanding drug metabolism, drug interactions, and personalized medicine strategies.
With the NR1I3 Transcription Factor Activity Assay kit, researchers can easily assess the activation or inhibition of NR1I3 in response to different compounds or experimental conditions. This kit is essential for studies focusing on drug development, toxicology, and pharmacogenomics, providing valuable insights into the mechanisms underlying drug metabolism and response variability.
| Product Name: | NR1I3 Transcription Factor Activity Assay |
| Product Code: | TFAB00178 |
| Target: | NR1I3 |
| Synonyms: | Constitutive activator of retinoid response, Constitutive active response, Constitutive androstane receptor, CAR, Orphan nuclear receptor MB67 |
| Reactivity: | Human |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie NR1I3 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor; DNA-binding Chromosomal Location of Human Ortholog: 1q23.3 Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; cytosol; nucleus Molecular Function:ligand-dependent nuclear receptor activity; androgen receptor activity; DNA binding; zinc ion binding; transcription coactivator activity; thyroid hormone receptor activity; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; androgen receptor signaling pathway; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q14994 |
| NCBI GenInfo Identifier: | 49066046 |
| NCBI Gene ID: | 9970 |
| NCBI Accession: | Q14994.2 |
| UniProt Secondary Accession: | Q14994,Q0VAC9, Q4U0F0, E9PB75, E9PC13, E9PDU3, E9PGH6 E9PH10, E9PHC8, E9PHN4, F1D8Q0, F1D8Q1, |
| UniProt Related Accession: | Q14994 |
| Molecular Weight: | 352 |
| NCBI Full Name: | Nuclear receptor subfamily 1 group I member 3 |
| NCBI Synonym Full Names: | nuclear receptor subfamily 1, group I, member 3 |
| NCBI Official Symbol: | NR1I3 |
| NCBI Official Synonym Symbols: | CAR; CAR1; MB67 |
| NCBI Protein Information: | nuclear receptor subfamily 1 group I member 3; constitutive active receptor; constitutive active response; orphan nuclear receptor MB67; orphan nuclear hormone receptor; constitutive androstane receptor; constitutive activator of retinoid response; constitutive androstane nuclear receptor variant 2; constitutive androstane nuclear receptor variant 3; constitutive androstane nuclear receptor variant 4; constitutive androstane nuclear receptor variant 5 |
| UniProt Protein Name: | Nuclear receptor subfamily 1 group I member 3 |
| UniProt Synonym Protein Names: | Constitutive activator of retinoid response; Constitutive active response; Constitutive androstane receptor; CAR; Orphan nuclear receptor MB67 |
| Protein Family: | Nuclear receptor subfamily |
| UniProt Gene Name: | NR1I3 |
| UniProt Entry Name: | NR1I3_HUMAN |
