MAFF Transcription Factor Activity Assay



ELISA Kit Technical Manual

MAFF Transcription Factor activity assay

Product Description:The Assay Genie MAFF transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates Assay Genie ELISA kits are designed to significantly reduce experiment Time: and ensure sensitivity and flexibility for high-throughput screening.
Target Synonyms:U-Maf;V-maf musculoaponeurotic fibrosarcoma oncogene homolog F
Detection Target:Phospho-IRAK1(Thr209)
Reactivity:Human, Mouse
Pack Size:12 x 8-Well Microstrips
Detection Method:Colorimetric 450 nm
Format:transcription factor activity assay
Storage and Stability Guarantee:4 °C/6 Months
Sample Type:Nuclear or cell lysates
Range:See Technical Manual
Time:4.5 hours

MAFF Transcription Factor activity assay Protein Information

UniProt Protein Function:MAFF: Interacts with the upstream promoter region of the oxytocin receptor gene. May be a transcriptional enhancer in the up-regulation of the oxytocin receptor gene at parturition. Since it lacks a putative transactivation domain, it may behave as a transcriptional repressor when it dimerize among himself. May also serve as a transcriptional activator by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. May be involved in the cellular stress response. Belongs to the bZIP family. Maf subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: mitochondrion; nucleoplasm; nucleus

Biological Process: blood coagulation; parturition; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

NCBI Summary:The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]
UniProt Code:Q9ULX9
NCBI GenInfo Identifier:21542145
NCBI Gene ID:23764
NCBI Accession:Q9ULX9.2
UniProt Secondary Accession:Q9ULX9,Q9Y525, B4DV49,
UniProt Related Accession:Q9ULX9
Molecular Weight:14,569 Da
NCBI Full Name:Transcription factor MafF
NCBI Synonym Full Names:MAF bZIP transcription factor F
NCBI Official Symbol:MAFF
NCBI Official Synonym Symbols:U-MAF; hMafF
NCBI Protein Information:transcription factor MafF
UniProt Protein Name:Transcription factor MafF
UniProt Synonym Protein Names:U-Maf; V-maf musculoaponeurotic fibrosarcoma oncogene homolog F
Protein Family:MaFF-interacting protein
UniProt Gene Name:MAFF
UniProt Entry Name:MAFF_HUMAN
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Additional Information

Product Type:
Transcription Factor Assay
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