Description
Fra-2 Transcription Factor Activity Assay
The FRA-2 Transcription Factor Activity Assay is a powerful tool for studying the activity of the FRA-2 transcription factor in various biological samples. This assay kit is designed to provide accurate and reliable measurements of FRA-2 activity, allowing researchers to investigate the role of this transcription factor in a wide range of cellular processes.FRA-2 is a key regulator of gene expression, controlling the transcription of genes involved in cell growth, differentiation, and survival. Dysregulation of FRA-2 activity has been implicated in various diseases, including cancer, inflammatory disorders, and metabolic conditions.
By measuring FRA-2 activity with this assay, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and identify potential targets for therapeutic intervention.The FRA-2 Transcription Factor Activity Assay offers high sensitivity and specificity, ensuring accurate measurements of FRA-2 activity in a variety of sample types. With its easy-to-use protocol and quick assay time, this kit is perfect for both experienced researchers and beginners looking to explore the functions of FRA-2 in their studies. Unlock the potential of FRA-2 research with this innovative assay kit from AssayGenie.
| Product Name: | Fra-2 Transcription Factor Activity Assay |
| Product Code: | TFAB00078 |
| Target: | Fra-2 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie Fra-2 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | FRA2: an oncogenic transcription factor of the bZIP family, Fos subfamily. The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity. Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate but short-lived, while FRA1 and FRA2 expression persists longer. FRA2 and JUND up-regulate the expression of CCR4, MYB, MDM2, and BCL6, and are expressed at high levels in CCR4-expressing mature T-cell malignancies such as adult T-cell leukemia/lymphoma (ATLL) and cutaneous T-cell lymphomas (CTCLs). May play a role in regulating the transcription of ICOS downstream of TCR/CD28 and cytokine receptor signaling. Its transcription is significantly increased in metastatic clear cell renal cell carcinomas. Belongs to the bZIP family, Fos subfamily. Three isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 2p23.3 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; transcription factor activity Biological Process: cell death; regulation of transcription from RNA polymerase II promoter |
| NCBI Summary: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq, Jul 2014] |
| UniProt Code: | P15408 |
| NCBI GenInfo Identifier: | 120487 |
| NCBI Gene ID: | 2355 |
| NCBI Accession: | P15408.1 |
| UniProt Secondary Accession: | P15408,Q6FG46, B2RD58, B3KP27, B4DYV4, |
| UniProt Related Accession: | P15408 |
| Molecular Weight: | 31,095 Da |
| NCBI Full Name: | Fos-related antigen 2 |
| NCBI Synonym Full Names: | FOS like 2, AP-1 transcription factor subunit |
| NCBI Official Symbol: | FOSL2 |
| NCBI Official Synonym Symbols: | FRA2 |
| NCBI Protein Information: | fos-related antigen 2 |
| UniProt Protein Name: | Fos-related antigen 2 |
| Protein Family: | Fos-related antigen |
| UniProt Gene Name: | FOSL2 |
| UniProt Entry Name: | FOSL2_HUMAN |
