Description
MEF2D (Phospho-Ser444)Transcription Factor Activity Assay
The MEF2D Phospho-Ser444 Transcription Factor Activity Assay offered by Assay Genie is a powerful tool for researchers studying transcription factor activity. This assay specifically detects phosphorylation of MEF2D at Ser444, providing valuable insights into the regulation of transcriptional activity.MEF2D is a key transcription factor involved in various cellular processes, including differentiation, proliferation, and survival. Phosphorylation at Ser444 is known to affect the activity of MEF2D, making it a critical site for studying its function.With high sensitivity and specificity, this assay delivers accurate and reproducible results, making it ideal for a wide range of research applications.
By understanding the activity of MEF2D and its phosphorylation status, researchers can gain valuable insights into gene regulation and potentially develop new therapies for diseases such as cancer, cardiovascular diseases, and neurodegenerative disorders.Overall, the MEF2D Phospho-Ser444 Transcription Factor Activity Assay is a valuable tool for researchers looking to advance their understanding of transcription factor activity and its role in various physiological and pathological processes.
| Product Name: | MEF2D (Phospho-Ser444)Transcription Factor Activity Assay |
| Product Code: | TFAB00055 |
| Target: | MEF2D (Phospho-Ser444) |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie MEF2D (Phospho-Ser444) transcription factor activity assay allows for the detection and qualitative analysis of MEF2D (Phospho-Ser444) active transcription factors in eukaryotic nuclear or cell lysates.
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | MEF2D: transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in muscle-specific and/or growth factor-related transcription. Six splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 1q12-q23 Cellular Component: cytoplasm; intracellular membrane-bound organelle; nucleoplasm; nucleus Molecular Function:histone deacetylase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription activator binding; transcription factor activity Biological Process: adult heart development; apoptosis; chondrocyte differentiation; endochondral ossification; muscle development; nervous system development; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] |
| UniProt Code: | Q14814 |
| NCBI GenInfo Identifier: | 2500876 |
| NCBI Gene ID: | 4209 |
| NCBI Accession: | Q14814.1 |
| UniProt Secondary Accession: | Q14814,Q14815, Q5T9U5, Q5T9U6, D3DVC0, |
| UniProt Related Accession: | Q14814 |
| Molecular Weight: | 49,856 Da |
| NCBI Full Name: | Myocyte-specific enhancer factor 2D |
| NCBI Synonym Full Names: | myocyte enhancer factor 2D |
| NCBI Official Symbol: | MEF2D |
| NCBI Protein Information: | myocyte-specific enhancer factor 2D |
| UniProt Protein Name: | Myocyte-specific enhancer factor 2D |
| Protein Family: | Myocyte-specific enhancer factor |
| UniProt Gene Name: | MEF2D |
| UniProt Entry Name: | MEF2D_HUMAN |
