Description
ATF1 Transcription Factor Activity Assay
The ATF1 Transcription Factor Activity Assay is a powerful tool for studying transcriptional regulation in cells. This assay allows researchers to measure the activity of ATF1, a key transcription factor involved in the regulation of gene expression.By quantifying ATF1 activity, researchers can gain valuable insights into the molecular mechanisms underlying various cellular processes, including cell proliferation, differentiation, and apoptosis.
The assay is highly sensitive and specific, providing accurate and reliable results for a wide range of research applications.Understanding the activity of ATF1 is essential for studying biological processes such as cancer, diabetes, and neurodegenerative disorders. By using the ATF1 Transcription Factor Activity Assay, researchers can uncover new therapeutic targets and develop potential treatments for these conditions.
| Product Name: | ATF1 Transcription Factor Activity Assay |
| Product Code: | TFAB00082 |
| Target: | ATF1 |
| Synonyms: | Activating transcription factor 1, Protein TREB36 |
| Reactivity: | Human, Mouse, Rat |
| Sample Types: | Nuclear or cell lysates |
The Assay Genie ATF1 transcription factor activity assay allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates
Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
| Assay Time: | 4.5 hours |
| Detection Method: | Colorimetric 450 nm |
| Size: | 12 x 8-Well Microstrips |
| Storage: | 4°C for 6 months |
| UniProt Protein Function: | ATF-1: a transcription factor that is a member of the leucine zipper family. Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Binds the Tax-responsive element (TRE) of HTLV-I. Activated downstream of IL-1. c-Src and TRAF6 are mediators of IL-1-induced AP-1 activation. Plays an important role in the trans-activation of the MHC class II trans-activator (CIITA) promoter III in B cells. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 12q13 Cellular Component: nucleoplasm; nucleus Molecular Function:protein binding; protein complex binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity Biological Process: cellular protein complex assembly; innate immune response; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of DNA replication; positive regulation of transcription from RNA polymerase II promoter; response to cobalt ion; response to organic cyclic substance; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes an activating transcription factor, which belongs to the ATF subfamily and bZIP (basic-region leucine zipper) family. It influences cellular physiologic processes by regulating the expression of downstream target genes, which are related to growth, survival, and other cellular activities. This protein is phosphorylated at serine 63 in its kinase-inducible domain by serine/threonine kinases, cAMP-dependent protein kinase A, calmodulin-dependent protein kinase I/II, mitogen- and stress-activated protein kinase and cyclin-dependent kinase 3 (cdk-3). Its phosphorylation enhances its transactivation and transcriptional activities, and enhances cell transformation. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in angiomatoid fibrous histiocytoma and clear cell sarcoma. This gene has a pseudogene on chromosome 6. [provided by RefSeq, Aug 2010] |
| UniProt Code: | P18846 |
| NCBI GenInfo Identifier: | 1168542 |
| NCBI Gene ID: | 466 |
| NCBI Accession: | P18846.2 |
| UniProt Secondary Accession: | P18846,P25168, Q9H4A8, B4DRF9, |
| UniProt Related Accession: | P18846,AAB25878 |
| Molecular Weight: | 15,295 Da |
| NCBI Full Name: | Cyclic AMP-dependent transcription factor ATF-1 |
| NCBI Synonym Full Names: | activating transcription factor 1 |
| NCBI Official Symbol: | ATF1 |
| NCBI Official Synonym Symbols: | TREB36; EWS-ATF1; FUS/ATF-1 |
| NCBI Protein Information: | cyclic AMP-dependent transcription factor ATF-1 |
| UniProt Protein Name: | Cyclic AMP-dependent transcription factor ATF-1 |
| UniProt Synonym Protein Names: | Activating transcription factor 1; Protein TREB36 |
| Protein Family: | Alcohol O-acetyltransferase |
| UniProt Gene Name: | ATF1 |
| UniProt Entry Name: | ATF1_HUMAN |
