Anti-CYB5A Antibody (CAB5401)

Antibody Name:	Anti-CYB5A Antibody
Antibody SKU:	CAB5401
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CYB5A (NP_683725.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, A-549, HT-29, 293T, M21

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human CYB5A (NP_683725.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEQ SDEA VKYY TLEE IQKH NHSK STWL ILHH KVYD LTKF LEEH PGGE EVLR EQAG GDAT ENFE DVGH STDA REMS KTFI IGEL HPDD RPKL NKPP ETLI
Gene ID:	1528
Uniprot:	P00167
Cellular Location:	Cytoplasm, Cytoplasmic side, Endoplasmic reticulum membrane, Microsome membrane, Single-pass membrane protein
Calculated MW:	11kDa/14kDa/15kDa
Observed MW:	17kDa

Synonyms:	CYB5A, CYB5, MCB5
Background:	The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 18q23 Cellular Component: endoplasmic reticulum membrane; mitochondrial outer membrane; membrane; integral to membrane Molecular Function:cytochrome-c oxidase activity; enzyme binding; metal ion binding; aldo-keto reductase activity; heme binding Biological Process: vitamin metabolic process; response to cadmium ion; L-ascorbic acid metabolic process; water-soluble vitamin metabolic process Disease: Methemoglobinemia Type Iv
NCBI Summary:	The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
UniProt Code:	P00167
NCBI GenInfo Identifier:	117809
NCBI Gene ID:	1528
NCBI Accession:	P00167.2
UniProt Secondary Accession:	P00167,Q6IB14, A8MV91, F8WEU4,
UniProt Related Accession:	P00167
Molecular Weight:	14,169 Da
NCBI Full Name:	Cytochrome b5
NCBI Synonym Full Names:	cytochrome b5 type A (microsomal)
NCBI Official Symbol:	CYB5A
NCBI Official Synonym Symbols:	CYB5; MCB5
NCBI Protein Information:	cytochrome b5; type 1 cyt-b5
UniProt Protein Name:	Cytochrome b5
UniProt Synonym Protein Names:	Microsomal cytochrome b5 type A; MCB5
Protein Family:	Cytochrome
UniProt Gene Name:	CYB5A
UniProt Entry Name:	CYB5_HUMAN

	Western blot analysis of extracts of various cell lines, using CYB5A antibody (CAB5401) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s.
	Immunohistochemistry of paraffin-embedded rat kidney using CYB5A antibody (CAB5401) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using CYB5A antibody (CAB5401) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using CYB5A Polyclonal Antibody (CAB5401) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
CYB5A Antibody (PACO00676)	Human Cytochrome b5 / CYB5A ELISA Kit
CYB5A Antibody (PACO08695)	Human CYB5A (Cytochrome b5) CLIA Kit (HUES00917)
	Human Cytochrome b5 (CYB5A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)