The Human Topoisomerase II Beta (Top2B) ELISA Kit is a powerful tool for accurately measuring levels of topoisomerase II beta in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable asset for a variety of research applications.Topoisomerase II beta is an essential enzyme involved in DNA replication, transcription, and chromosome segregation. Dysregulation of topoisomerase II beta has been implicated in various diseases, including cancer, autoimmune disorders, and neurodegenerative diseases.
Therefore, studying its levels can provide insights into disease mechanisms and potential therapeutic targets.This ELISA kit is user-friendly and easy to use, allowing researchers to obtain accurate measurements of topoisomerase II beta levels in a fast and efficient manner. Overall, the Human Topoisomerase II Beta (Top2B) ELISA Kit is a valuable tool for researchers studying the role of this enzyme in health and disease.
Product Name:
Human Topoisomerase II Beta (TOP2b) ELISA Kit
Product Code:
HUDL02960
Size:
96 Assays
Target:
TOP2b
Synonyms:
TOPIIB, Top2beta, DNA topoisomerase II, beta isozyme
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
