The Human RAD54-like protein 2 (RAD54L2) ELISA Kit from Assay Genie is a powerful tool for accurately measuring RAD54L2 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring precise and reproducible results for a wide range of research applications.RAD54L2 is a critical protein involved in DNA repair and maintenance, playing a key role in genomic stability and preventing mutations that can lead to diseases such as cancer.
By measuring RAD54L2 levels, researchers can gain valuable insights into DNA repair processes and potential implications for disease development.Discover the potential of RAD54L2 as a biomarker for studying DNA repair mechanisms and its role in disease progression with the Human RAD54L2 ELISA Kit from Assay Genie. Trust in its reliability and accuracy for advancing your research in genetics, oncology, and beyond.
Product Name:
Human RAD54 Like Protein 2 (RAD54L2) ELISA Kit
Product Code:
HUDL02493
Size:
96 Assays
Target:
RAD54L2
Synonyms:
SRISNF2L, Helicase ARIP4, Androgen receptor-interacting protein 4
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
