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Anti-UBE2F Antibody (CAB5160)

SKU:
CAB5160
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 2 Anti-UBE2F Antibody CAB5160
  • Signal Transduction Antibodies 2 Anti-UBE2F Antibody CAB5160
  • Signal Transduction Antibodies 2 Anti-UBE2F Antibody CAB5160
  • Signal Transduction Antibodies 2 Anti-UBE2F Antibody CAB5160
  • Signal Transduction Antibodies 2 Anti-UBE2F Antibody CAB5160
€139 - €419
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Description

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Antibody Name:Anti-UBE2F Antibody
Antibody SKU:CAB5160
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:K-562, Mouse liver, Mouse heart, Mouse pancreas, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human UBE2F (NP_542409.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MLTL ASKL KRDD GLKG SRTA ATAS DSTR RVSV RDKL LVKE VAEL EANL PCTC KVHF PDPN KLHC FQLT VTPD EGYY QGGK FQFE TEVP DAYN MVPP KVKC LTKI WHPN ITET GEIC LSLL REHS IDGT GWAP TRTL KDVV WGLN SLFT DLLN FDDP LNIE AAEH HLRD KEDF RNKV DDYI KRYA R
Gene ID:140739
Uniprot:Q969M7
Cellular Location:
Calculated MW:11kDa/13kDa/17kDa/18kDa/21kDa/23kDa
Observed MW:21kDa
Synonyms:UBE2F, NCE2
Background:
UniProt Protein Function:UBE2F: Accepts the ubiquitin-like protein NEDD8 from the UBA3- NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 6.3.2.19; Ubiquitin ligase; EC 6.3.2.-; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: cytoplasm

Molecular Function:protein binding; ubiquitin protein ligase binding; ATP binding; NEDD8 ligase activity; ligase activity

Biological Process: protein neddylation; protein ubiquitination

UniProt Code:Q969M7
NCBI GenInfo Identifier:74751725
NCBI Gene ID:140739
NCBI Accession:Q969M7.1
UniProt Related Accession:Q969M7
Molecular Weight:Predicted Molecular Mass: 24.8kDaAccurate Molecular Mass: 25kDa
NCBI Full Name:NEDD8-conjugating enzyme UBE2F
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 F (putative)
NCBI Official Symbol:UBE2F  
NCBI Official Synonym Symbols:NCE2  
NCBI Protein Information:NEDD8-conjugating enzyme UBE2F
UniProt Protein Name:NEDD8-conjugating enzyme UBE2F
UniProt Synonym Protein Names:NEDD8 carrier protein UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; Ubiquitin-conjugating enzyme E2 F
Protein Family:NEDD8-conjugating enzyme
UniProt Gene Name:UBE2F  
UniProt Entry Name:UBE2F_HUMAN
Anti-UBE2F Antibody (CAB5160)Western blot analysis of extracts of various cell lines, using UBE2F antibody (CAB5160) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-UBE2F Antibody (CAB5160)Immunohistochemistry of paraffin-embedded rat ovary using UBE2F Rabbit pAb (CAB5160) at dilution of 1:200 (40x lens).
Anti-UBE2F Antibody (CAB5160)Immunohistochemistry of paraffin-embedded human liver cancer using UBE2F Rabbit pAb (CAB5160) at dilution of 1:200 (40x lens).
Anti-UBE2F Antibody (CAB5160)Immunohistochemistry of paraffin-embedded mouse heart using UBE2F Rabbit pAb (CAB5160) at dilution of 1:200 (40x lens).
Anti-UBE2F Antibody (CAB5160)Immunofluorescence analysis of NIH-3T3 cells using UBE2F antibody (CAB5160) at dilution of 1:100. Blue: DAPI for nuclear staining.

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