Anti-UBE2H Antibody (CAB7344)

Antibody Name:	Anti-UBE2H Antibody
Antibody SKU:	CAB7344
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, BT-474, 293T, U-251MG, SKOV3, Mouse thymus, Mouse liver, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human UBE2H (NP_003335.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSP SPGK RRMD TDVV KLIE SKHE VTIL GGLN EFVV KFYG PQGT PYEG GVWK VRVD LPDK YPFK SPSI GFMN KIFH PNID EASG TVCL DVIN QTWT ALYD LTNI FESF LPQL LAYP NPID PLNG DAAA MYLH RPEE YKQK IKEY IQKY ATEE ALKE QEEG TGDS SSES SMSD FSED EAQD MEL
Gene ID:	7328
Uniprot:	P62256
Cellular Location:
Calculated MW:	17kDa/20kDa
Observed MW:	21-42kDa

Synonyms:

UBE2H, E2-20K, GID3, UBC8, UBCH, UBCH2

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

UniProt Protein Function:	UBE2H: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A. Belongs to the ubiquitin-conjugating enzyme family.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; EC 6.3.2.19; Mitochondrial; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: 7q32 Cellular Component: cytoplasm Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process
NCBI Summary:	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq, Feb 2011]
UniProt Code:	P62256
NCBI GenInfo Identifier:	51338683
NCBI Gene ID:	7328
NCBI Accession:	P62256.1
UniProt Secondary Accession:	P62256,P37286, Q7Z6F4, A4D1L6, C9JY93,
UniProt Related Accession:	P62256
Molecular Weight:	183
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 H
NCBI Synonym Full Names:	ubiquitin-conjugating enzyme E2H
NCBI Official Symbol:	UBE2H
NCBI Official Synonym Symbols:	GID3; UBC8; UBCH; UBCH2; E2-20K
NCBI Protein Information:	ubiquitin-conjugating enzyme E2 H; ubiquitin-protein ligase H; ubiquitin carrier protein H; GID complex subunit 3, UBC8 homolog; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8)
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 H
UniProt Synonym Protein Names:	UbcH2; Ubiquitin carrier protein H; Ubiquitin-conjugating enzyme E2-20K; Ubiquitin-protein ligase H
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2H
UniProt Entry Name:	UBE2H_HUMAN

	Western blot analysis of extracts of various cell lines, using UBE2H antibody (CAB7344) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using UBE2H antibody (CAB7344). Blue: DAPI for nuclear staining.

Antibodies

UBE2H Antibody (PACO13031)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)