The MPO Antibody (CAB1374) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity and sensitivity for detecting MPO in human samples, making it ideal for use in various research applications such as Western blot and immunohistochemistry.MPO is an enzyme involved in the immune response, playing a crucial role in the defense against pathogens by generating reactive oxygen species. Abnormalities in MPO activity have been implicated in various inflammatory and autoimmune diseases, making it a valuable target for research.
This antibody is validated for use in WB, IHC-P, ELISA, IF-P applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MPO Antibody
SKU:
CAB1374
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MPO, Myeloperoxidase (MPO)
Cellular Localization:
Lysosome.
Calculated MW:
84kDa
Observed MW:
60kDa
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.
Purification Method
Affinity purification
Gene ID
4353
RRID
AB_2760599
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Myeloperoxidase (MPO) Rabbit pAb (CAB1374) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Myeloperoxidase (MPO) Rabbit pAb (CAB1374) at a dilution of 1:4000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence analysis of rat bone marrow using Myeloperoxidase (MPO) Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse bone marrow using Myeloperoxidase (MPO) Rabbit pAb (CAB1374) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
