Anti-PRPF8 Antibody (CAB6053)

Antibody Name:	Anti-PRPF8 Antibody
Antibody SKU:	CAB6053
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Jurkat

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PRPF8 (NP_006436.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGV FPYR GPGN PVPG PLAP LPDY MSEE KLQE KARK WQQL QAKR YAEK RKFG FVDA QKED MPPE HVRK IIRD HGDM TNRK FRHD KRVY LGAL KYMP HAVL
Gene ID:	10594
Uniprot:	Q6P2Q9
Cellular Location:	Nucleus speckle
Calculated MW:	273kDa
Observed MW:	274kDa

Synonyms:

PRPF8, HPRP8, PRP8, PRPC8, RP13, SNRNP220, PRPF8

Background:

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

UniProt Protein Function:	Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
NCBI Summary:	Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]
UniProt Code:	Q6P2Q9
NCBI GenInfo Identifier:	67460824
NCBI Gene ID:	10594
NCBI Accession:	Q6P2Q9.2
UniProt Related Accession:	Q6P2Q9
Molecular Weight:
NCBI Full Name:	Pre-mRNA-processing-splicing factor 8
NCBI Synonym Full Names:	pre-mRNA processing factor 8
NCBI Official Symbol:	PRPF8
NCBI Official Synonym Symbols:	PRP8; RP13; HPRP8; PRPC8; SNRNP220
NCBI Protein Information:	pre-mRNA-processing-splicing factor 8
UniProt Protein Name:	Pre-mRNA-processing-splicing factor 8
UniProt Synonym Protein Names:	220 kDa U5 snRNP-specific protein; PRP8 homolog; Splicing factor Prp8; p220
Protein Family:	Pre-mRNA-splicing factor
UniProt Gene Name:	PRPF8

	Western blot analysis of extracts of various cell lines, using PRPF8 antibody (CAB6053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody (CAB6053) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody (CAB6053) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody (CAB6053) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-251MG cells using PRPF8 antibody (CAB6053) at dilution of 1:100 (20x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-PRPF8 Antibody (CAB4575)

PRPF8 Antibody (PACO11500)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)